Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand

Zanthoxylum limonella (Dennst.) Alston. (Rutaceae) or Ma-khwaen is one of the medicinal plants in Thai traditional medicine. To investigate the pharmacognostic specifications and chemical constituents of Z. limonella fruits and seeds. Fruits and seeds of Z. limonella were collected from 15 sources t...

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Main Authors: Rawiwan Charoensup, Thidarat Duangyod, Pravaree Phuneerub, Chatubhong Singharachai
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2016-01-01
Series:Journal of Advanced Pharmaceutical Technology & Research
Subjects:
Online Access:http://www.japtr.org/article.asp?issn=2231-4040;year=2016;volume=7;issue=4;spage=134;epage=138;aulast=Charoensup
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spelling doaj-d552847172964b86850e99a37ae29c1f2020-11-24T23:33:13ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942016-01-017413413810.4103/2231-4040.191421Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in ThailandRawiwan CharoensupThidarat DuangyodPravaree PhuneerubChatubhong SingharachaiZanthoxylum limonella (Dennst.) Alston. (Rutaceae) or Ma-khwaen is one of the medicinal plants in Thai traditional medicine. To investigate the pharmacognostic specifications and chemical constituents of Z. limonella fruits and seeds. Fruits and seeds of Z. limonella were collected from 15 sources throughout Thailand; then examined the pharmacognostic specification following WHO guideline of quality control method for medicinal plant materials. Microscopic determination of Z. limonella powders demonstrated fragment of mesocarp, fragment of brown vitta, oil glands, fragment of endocarp, and endosperm containing oil globule, trichome and pale brown stone cells. Stomatal index and pellucid dots in mm 2 were 19.87 and 4.2 respectively. Physico-chemical parameters unveiled that loss on drying, water content, total ash, and acid-insoluble ash should be not >17.90%, 9.18%, 4.50%, and 0.60% of dried weight respectively; while ethanol, water, and hexane extractive values and volatile oil content should be not <2.24%, 2.27%, 1.57% and 9.63% of dried weight respectively. R f values of thin-layer chromatographic fingerprint of Z. limonella fruits and seeds ethanolic extract were 0.38, 0.45, 0.90, and 0.97 detected ultraviolet (UV) light 254 nm, 0.30, 0.44, 0.67, and 0.77 detected UV light 366 nm, and 0.24, 0.73, 0.78, and 0.93 detected 10% sulfuric acid. There are three main chemical compounds in Z. limonella oil including limonene (43.63%), (+)-sabinene (16.72%), and terpinen-4-ol (10.95%). The result gained from pharmacognostic specifications and chemical fingerprints could be used as standardization data of Z. limonella fruits and seeds to apply or provide for guarantee of quality.http://www.japtr.org/article.asp?issn=2231-4040;year=2016;volume=7;issue=4;spage=134;epage=138;aulast=CharoensupGas chromatography/mass spectrometrypharmacognostic specificationphysico-chemical parameterthin-layer chromatographic fingerprintZanthoxylum limonella
collection DOAJ
language English
format Article
sources DOAJ
author Rawiwan Charoensup
Thidarat Duangyod
Pravaree Phuneerub
Chatubhong Singharachai
spellingShingle Rawiwan Charoensup
Thidarat Duangyod
Pravaree Phuneerub
Chatubhong Singharachai
Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand
Journal of Advanced Pharmaceutical Technology & Research
Gas chromatography/mass spectrometry
pharmacognostic specification
physico-chemical parameter
thin-layer chromatographic fingerprint
Zanthoxylum limonella
author_facet Rawiwan Charoensup
Thidarat Duangyod
Pravaree Phuneerub
Chatubhong Singharachai
author_sort Rawiwan Charoensup
title Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand
title_short Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand
title_full Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand
title_fullStr Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand
title_full_unstemmed Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand
title_sort pharmacognostic specification of zanthoxylum limonella (dennst.) alston: fruits and seeds in thailand
publisher Wolters Kluwer Medknow Publications
series Journal of Advanced Pharmaceutical Technology & Research
issn 2231-4040
0976-2094
publishDate 2016-01-01
description Zanthoxylum limonella (Dennst.) Alston. (Rutaceae) or Ma-khwaen is one of the medicinal plants in Thai traditional medicine. To investigate the pharmacognostic specifications and chemical constituents of Z. limonella fruits and seeds. Fruits and seeds of Z. limonella were collected from 15 sources throughout Thailand; then examined the pharmacognostic specification following WHO guideline of quality control method for medicinal plant materials. Microscopic determination of Z. limonella powders demonstrated fragment of mesocarp, fragment of brown vitta, oil glands, fragment of endocarp, and endosperm containing oil globule, trichome and pale brown stone cells. Stomatal index and pellucid dots in mm 2 were 19.87 and 4.2 respectively. Physico-chemical parameters unveiled that loss on drying, water content, total ash, and acid-insoluble ash should be not >17.90%, 9.18%, 4.50%, and 0.60% of dried weight respectively; while ethanol, water, and hexane extractive values and volatile oil content should be not <2.24%, 2.27%, 1.57% and 9.63% of dried weight respectively. R f values of thin-layer chromatographic fingerprint of Z. limonella fruits and seeds ethanolic extract were 0.38, 0.45, 0.90, and 0.97 detected ultraviolet (UV) light 254 nm, 0.30, 0.44, 0.67, and 0.77 detected UV light 366 nm, and 0.24, 0.73, 0.78, and 0.93 detected 10% sulfuric acid. There are three main chemical compounds in Z. limonella oil including limonene (43.63%), (+)-sabinene (16.72%), and terpinen-4-ol (10.95%). The result gained from pharmacognostic specifications and chemical fingerprints could be used as standardization data of Z. limonella fruits and seeds to apply or provide for guarantee of quality.
topic Gas chromatography/mass spectrometry
pharmacognostic specification
physico-chemical parameter
thin-layer chromatographic fingerprint
Zanthoxylum limonella
url http://www.japtr.org/article.asp?issn=2231-4040;year=2016;volume=7;issue=4;spage=134;epage=138;aulast=Charoensup
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AT pravareephuneerub pharmacognosticspecificationofzanthoxylumlimonelladennstalstonfruitsandseedsinthailand
AT chatubhongsingharachai pharmacognosticspecificationofzanthoxylumlimonelladennstalstonfruitsandseedsinthailand
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