Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia

• Main Authors: Rajković Dragana, Taški-Ajduković Ksenija, Nagl Nevena, Isakov Milada, Milić Dragan, Karagić Đura, Mikić Aleksandar
• Format: Article
• Language: English
• Published: Institute of field and vegetable crops, Novi Sad 2015-01-01
• Series: Ratarstvo i Povrtarstvo
• Subjects: DNA polymorphism; ISSRs; molecular markers; SSRs; Vicia
• Online Access: http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2015/1821-39441502085R.pdf

The development of novel markers usually requires high costs and is time consuming. Instead of developing new markers, their transfer within a genus is an alternative particularly suitable in less studied species. Hence the goal of this study was to evaluate the ability of SSR markers, previously developed from Vicia faba, and ISSR markers to estimate DNA polymorphism within the genus Vicia. Eight Vicia species were used for cross-species amplification. Transferability of SSR markers in the studied species ranged from 25% to 100%, with an average of 56.25%. Although three SSR primers were polymorphic, the nature of the amplification products indicated that their further usefulness is questionable. ISSR primers generated polymorphic products of amplification in all tested Vicia species. The average polymorphism of ISSR primers was 93.75%, which implies that these primers can be used within the genus Vicia.