Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.

Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.

RNA Polymerase II pauses and backtracks during transcription, with many consequences for gene expression and cellular physiology. Here, we show that the energy required to melt double-stranded nucleic acids in the transcription bubble predicts pausing in Saccharomyces cerevisiae far more accurately...

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Bibliographic Details
Main Authors: Martin Lukačišin, Matthieu Landon, Rishi Jajoo
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5354634?pdf=render
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spelling doaj-d54281bb6e194d60be5c6afbea8e546a2020-11-25T01:14:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017406610.1371/journal.pone.0174066Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.Martin LukačišinMatthieu LandonRishi JajooRNA Polymerase II pauses and backtracks during transcription, with many consequences for gene expression and cellular physiology. Here, we show that the energy required to melt double-stranded nucleic acids in the transcription bubble predicts pausing in Saccharomyces cerevisiae far more accurately than nucleosome roadblocks do. In addition, the same energy difference also determines when the RNA polymerase backtracks instead of continuing to move forward. This data-driven model corroborates-in a genome wide and quantitative manner-previous evidence that sequence-dependent thermodynamic features of nucleic acids influence both transcriptional pausing and backtracking.http://europepmc.org/articles/PMC5354634?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Martin Lukačišin
Matthieu Landon
Rishi Jajoo
spellingShingle Martin Lukačišin
Matthieu Landon
Rishi Jajoo
Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.
PLoS ONE
author_facet Martin Lukačišin
Matthieu Landon
Rishi Jajoo
author_sort Martin Lukačišin
title Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.
title_short Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.
title_full Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.
title_fullStr Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.
title_full_unstemmed Sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.
title_sort sequence-specific thermodynamic properties of nucleic acids influence both transcriptional pausing and backtracking in yeast.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2017-01-01
description RNA Polymerase II pauses and backtracks during transcription, with many consequences for gene expression and cellular physiology. Here, we show that the energy required to melt double-stranded nucleic acids in the transcription bubble predicts pausing in Saccharomyces cerevisiae far more accurately than nucleosome roadblocks do. In addition, the same energy difference also determines when the RNA polymerase backtracks instead of continuing to move forward. This data-driven model corroborates-in a genome wide and quantitative manner-previous evidence that sequence-dependent thermodynamic features of nucleic acids influence both transcriptional pausing and backtracking.
url http://europepmc.org/articles/PMC5354634?pdf=render
work_keys_str_mv AT martinlukacisin sequencespecificthermodynamicpropertiesofnucleicacidsinfluencebothtranscriptionalpausingandbacktrackinginyeast
AT matthieulandon sequencespecificthermodynamicpropertiesofnucleicacidsinfluencebothtranscriptionalpausingandbacktrackinginyeast
AT rishijajoo sequencespecificthermodynamicpropertiesofnucleicacidsinfluencebothtranscriptionalpausingandbacktrackinginyeast
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