Overcoming crossing barriers between jatropha (Jatropha curcas L.) and castor bean (Ricinus communisL.)

Overcoming crossing barriers between jatropha (Jatropha curcas L.) and castor bean (Ricinus communisL.)

A limitation in improvement of jatropha (Jatropha curcas) has been the low genetic variation among the available germplasm. An alternative to create genetic variability and incorporate desirable traits is through wide crossing. Due to reproductive barriers, wide crossing in jatropha has had limited...

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Main Authors: Kularb Laosatit, Naratid Mokrong, Patcharin Tanya, Peerasak Srinives
Format: Article
Language:English
Published: Brazilian Society of Plant Breeding 2017-04-01
Series:Crop Breeding and Applied Biotechnology
Subjects:
Intergeneric hybrid
post-fertilization barrier
embryo rescue
ovule culture
Online Access:http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-a82f-f60c.pdf
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spelling doaj-d500d4c094d64492b081c5e5fad45cdb2020-11-25T01:29:51ZengBrazilian Society of Plant BreedingCrop Breeding and Applied Biotechnology1518-78531984-70332017-04-0117216416710.1590/1984-70332017v17n2n24Overcoming crossing barriers between jatropha (Jatropha curcas L.) and castor bean (Ricinus communisL.)Kularb Laosatit0Naratid Mokrong1Patcharin Tanya2Peerasak Srinives3 Kasetsart University, Faculty of Agriculture at Kamphaeng Saen, Department of Agronomy, Kamphaeng Saen, NakhonPathom 73140, ThailandPrincess Naradhiwas University, Faculty of Agriculture, Narathiwat 96110, Thailand, Kasetsart University, Faculty of Agriculture at Kamphaeng Saen, Department of Agronomy, Kamphaeng Saen, NakhonPathom 73140, Thailand Kasetsart University, Faculty of Agriculture at Kamphaeng Saen, Department of Agronomy, Kamphaeng Saen, NakhonPathom 73140, ThailandA limitation in improvement of jatropha (Jatropha curcas) has been the low genetic variation among the available germplasm. An alternative to create genetic variability and incorporate desirable traits is through wide crossing. Due to reproductive barriers, wide crossing in jatropha has had limited success. In this experiment, intergeneric hybridization between jatropha and castor bean (Ricinus communis) was performed. In both direct and reciprocal crosses, a few stigma-compatible pollen grains were found. The pollen tube grew normally and reached the style base within an hour after pollination, but the embryo aborted a few days later. This indicated that the hybridization barrier was post-fertilization and thus the ovules were excised, cultured in MS medium supplemented with 1 mg L-1 IAA, and subcultured every four weeks. Finally we obtained one intergeneric hybrid plant, which was vegetatively propagated for further study.http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-a82f-f60c.pdfIntergeneric hybridpost-fertilization barrierembryo rescueovule culture
collection DOAJ
language English
format Article
sources DOAJ
author Kularb Laosatit
Naratid Mokrong
Patcharin Tanya
Peerasak Srinives
spellingShingle Kularb Laosatit
Naratid Mokrong
Patcharin Tanya
Peerasak Srinives
Overcoming crossing barriers between jatropha (Jatropha curcas L.) and castor bean (Ricinus communisL.)
Crop Breeding and Applied Biotechnology
Intergeneric hybrid
post-fertilization barrier
embryo rescue
ovule culture
author_facet Kularb Laosatit
Naratid Mokrong
Patcharin Tanya
Peerasak Srinives
author_sort Kularb Laosatit
title Overcoming crossing barriers between jatropha (Jatropha curcas L.) and castor bean (Ricinus communisL.)
title_short Overcoming crossing barriers between jatropha (Jatropha curcas L.) and castor bean (Ricinus communisL.)
title_full Overcoming crossing barriers between jatropha (Jatropha curcas L.) and castor bean (Ricinus communisL.)
title_fullStr Overcoming crossing barriers between jatropha (Jatropha curcas L.) and castor bean (Ricinus communisL.)
title_full_unstemmed Overcoming crossing barriers between jatropha (Jatropha curcas L.) and castor bean (Ricinus communisL.)
title_sort overcoming crossing barriers between jatropha (jatropha curcas l.) and castor bean (ricinus communisl.)
publisher Brazilian Society of Plant Breeding
series Crop Breeding and Applied Biotechnology
issn 1518-7853
1984-7033
publishDate 2017-04-01
description A limitation in improvement of jatropha (Jatropha curcas) has been the low genetic variation among the available germplasm. An alternative to create genetic variability and incorporate desirable traits is through wide crossing. Due to reproductive barriers, wide crossing in jatropha has had limited success. In this experiment, intergeneric hybridization between jatropha and castor bean (Ricinus communis) was performed. In both direct and reciprocal crosses, a few stigma-compatible pollen grains were found. The pollen tube grew normally and reached the style base within an hour after pollination, but the embryo aborted a few days later. This indicated that the hybridization barrier was post-fertilization and thus the ovules were excised, cultured in MS medium supplemented with 1 mg L-1 IAA, and subcultured every four weeks. Finally we obtained one intergeneric hybrid plant, which was vegetatively propagated for further study.
topic Intergeneric hybrid
post-fertilization barrier
embryo rescue
ovule culture
url http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-a82f-f60c.pdf
work_keys_str_mv AT kularblaosatit overcomingcrossingbarriersbetweenjatrophajatrophacurcaslandcastorbeanricinuscommunisl
AT naratidmokrong overcomingcrossingbarriersbetweenjatrophajatrophacurcaslandcastorbeanricinuscommunisl
AT patcharintanya overcomingcrossingbarriersbetweenjatrophajatrophacurcaslandcastorbeanricinuscommunisl
AT peerasaksrinives overcomingcrossingbarriersbetweenjatrophajatrophacurcaslandcastorbeanricinuscommunisl
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