Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing

Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing

In this work, the surface of nanostructured fluorine-doped ZnO (nZnO·F) is functionalized with protein A (PrA), and used as a model biomolecule. The chemical procedure is characterized by several analytical techniques such as Fourier Transform Infrared Spectroscopy, water contact angle anal...

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Main Authors: Giovanna Chianese, Monica Terracciano, Rosalba Moretta, Paolo Cappiello, Giuseppe Vitiello, Antonio Aronne, Chiara Schiattarella, Luca De Stefano, Ilaria Rea
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:Applied Sciences
Subjects:
nanostructured zinc oxide
hydrothermal synthesis
bioconjugation
photoluminescence
Online Access:https://www.mdpi.com/2076-3417/9/16/3380
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spelling doaj-d4e86f29a7c4435c84d3e98fd174db282020-11-24T21:34:18ZengMDPI AGApplied Sciences2076-34172019-08-01916338010.3390/app9163380app9163380Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical BiosensingGiovanna Chianese0Monica Terracciano1Rosalba Moretta2Paolo Cappiello3Giuseppe Vitiello4Antonio Aronne5Chiara Schiattarella6Luca De Stefano7Ilaria Rea8National Research Council, Institute for Microelectronics and Microsystems, Unit of Naples, 80131 Naples, ItalyNational Research Council, Institute for Microelectronics and Microsystems, Unit of Naples, 80131 Naples, ItalyNational Research Council, Institute for Microelectronics and Microsystems, Unit of Naples, 80131 Naples, ItalyNational Research Council, Institute for Microelectronics and Microsystems, Unit of Naples, 80131 Naples, ItalyDepartment of Chemical, Materials and Production Engineering, Federico II University of Naples, 80138 Naples, ItalyDepartment of Chemical, Materials and Production Engineering, Federico II University of Naples, 80138 Naples, ItalyNational Research Council, Institute for Microelectronics and Microsystems, Unit of Naples, 80131 Naples, ItalyNational Research Council, Institute for Microelectronics and Microsystems, Unit of Naples, 80131 Naples, ItalyNational Research Council, Institute for Microelectronics and Microsystems, Unit of Naples, 80131 Naples, ItalyIn this work, the surface of nanostructured fluorine-doped ZnO (nZnO·F) is functionalized with protein A (PrA), and used as a model biomolecule. The chemical procedure is characterized by several analytical techniques such as Fourier Transform Infrared Spectroscopy, water contact angle analysis, and fluorescence microscopy. The surface modification of nZnO·F by binding increasing concentrations of PrA is also investigated by two label-free optical techniques, i.e., the spectroscopic reflectometry and the steady-state photoluminescence. The results are compared with those obtained using undoped nZnO substrates in order to highlight the better performances of nZnO·F due to the fluorine doping. The results of this study pave the way for the design and realization of a ZnO-based nanostructured platform for label-free optical sensing.https://www.mdpi.com/2076-3417/9/16/3380nanostructured zinc oxidehydrothermal synthesisbioconjugationphotoluminescence
collection DOAJ
language English
format Article
sources DOAJ
author Giovanna Chianese
Monica Terracciano
Rosalba Moretta
Paolo Cappiello
Giuseppe Vitiello
Antonio Aronne
Chiara Schiattarella
Luca De Stefano
Ilaria Rea
spellingShingle Giovanna Chianese
Monica Terracciano
Rosalba Moretta
Paolo Cappiello
Giuseppe Vitiello
Antonio Aronne
Chiara Schiattarella
Luca De Stefano
Ilaria Rea
Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing
Applied Sciences
nanostructured zinc oxide
hydrothermal synthesis
bioconjugation
photoluminescence
author_facet Giovanna Chianese
Monica Terracciano
Rosalba Moretta
Paolo Cappiello
Giuseppe Vitiello
Antonio Aronne
Chiara Schiattarella
Luca De Stefano
Ilaria Rea
author_sort Giovanna Chianese
title Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing
title_short Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing
title_full Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing
title_fullStr Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing
title_full_unstemmed Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing
title_sort synthesis and surface modification of nanostructured f-doped zno: toward a transducer for label-free optical biosensing
publisher MDPI AG
series Applied Sciences
issn 2076-3417
publishDate 2019-08-01
description In this work, the surface of nanostructured fluorine-doped ZnO (nZnO·F) is functionalized with protein A (PrA), and used as a model biomolecule. The chemical procedure is characterized by several analytical techniques such as Fourier Transform Infrared Spectroscopy, water contact angle analysis, and fluorescence microscopy. The surface modification of nZnO·F by binding increasing concentrations of PrA is also investigated by two label-free optical techniques, i.e., the spectroscopic reflectometry and the steady-state photoluminescence. The results are compared with those obtained using undoped nZnO substrates in order to highlight the better performances of nZnO·F due to the fluorine doping. The results of this study pave the way for the design and realization of a ZnO-based nanostructured platform for label-free optical sensing.
topic nanostructured zinc oxide
hydrothermal synthesis
bioconjugation
photoluminescence
url https://www.mdpi.com/2076-3417/9/16/3380
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