Comparison Between Antifungal Activitiesof Xenorhabdus stockiae PB09 Cell-free Supernatants Derivedfrom Shake-Flask CultivationandFermentation

• Main Author: Prapassorn Bussaman
• Format: Article
• Language: Thai
• Published: Mahasarakham University 2020-04-01
• Series: Warasan Witthayasat Lae Theknoloyi Mahawitthayalai Mahasarakham
• Subjects: xenorhabdus stockiae; antifungal activity; colletotrichum gloeosporioides
• Online Access: http://journal.msu.ac.th/upload/articles/article2602_19934.pdf

A varietyof metabolites from the entomopathogenic bacterium Xenorhabdus spp. have been reported to have antifungal activities, especially against Colletotrichum gloeosporioides (Penz.) Sacc., which is one of the most damaging pathogens targeting many tropical fruit plants by causing anthracnose disease. This study aimed to evaluate and compare the antifungal activities of Xenorhabdus stockiae PB09 cell-free supernatants derived from cultivations in shake-flask and fermenter scales for controlling C. gloeosporioides mycelial growth by using poisoned food technique on agar media. The results showed that cell-free supernatants of both shake flask-scale and fermenter-scale productions could inhibit the growth of C.gloeosporioides. Antifungal activities of X. stockiae PB09cell frees upernatants derived from cultivations by shake-flasks and 5L fermenters increased over time and reached their peaks at 72 and 48 h, respectively. Fermentation also resulted in the levels of antifungal activities of X. stockiae PB09 cell-free supernatant being higher than that obtained by shake-flask cultivation. This study demonstrated that cell-free supernatant of X. stockiae PB09 could be used to control the growth of C. gloeosporioides and the large scale production using fermenter was superior to shake flask cultivation by giving more effective cell-free supernatant in a shorter period of time for up to 24 h.