Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs.
Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed...
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2020-12-01
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Online Access: | https://doi.org/10.1371/journal.pbio.3000879 |
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doaj-d4d0c9db78524b23a0be687787c9ab2f2021-07-02T17:09:57ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852020-12-011812e300087910.1371/journal.pbio.3000879Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs.Antony FearnsDaniel J GreenwoodAngela RodgersHaibo JiangMaximiliano G GutierrezCorrelative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type-specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, we found that the anti-tuberculosis (TB) drug bedaquiline (BDQ) is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells.https://doi.org/10.1371/journal.pbio.3000879 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Antony Fearns Daniel J Greenwood Angela Rodgers Haibo Jiang Maximiliano G Gutierrez |
spellingShingle |
Antony Fearns Daniel J Greenwood Angela Rodgers Haibo Jiang Maximiliano G Gutierrez Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs. PLoS Biology |
author_facet |
Antony Fearns Daniel J Greenwood Angela Rodgers Haibo Jiang Maximiliano G Gutierrez |
author_sort |
Antony Fearns |
title |
Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs. |
title_short |
Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs. |
title_full |
Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs. |
title_fullStr |
Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs. |
title_full_unstemmed |
Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs. |
title_sort |
correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in mycobacterium tuberculosis-infected lungs. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Biology |
issn |
1544-9173 1545-7885 |
publishDate |
2020-12-01 |
description |
Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type-specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, we found that the anti-tuberculosis (TB) drug bedaquiline (BDQ) is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells. |
url |
https://doi.org/10.1371/journal.pbio.3000879 |
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