The Study of the sensory threshold of pain in male rats born to normal mothers and mothers with metabolic alkalosis by formalin test

• Main Authors: n nariman, s khatamsaz, E Sharifi
• Format: Article
• Language: fas
• Published: Yasuj University Of Medical Sciences 2015-07-01
• Series: Armaghane Danesh Bimonthly Journal
• Subjects: sensory thresholds of pain; pain score; metabolic alkalosis; rat
• Online Access: http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-98-1&slc_lang=en&sid=1

Background & aim: metabolic alkalosis is one of the most common disorders of the acid-base body fluids that followed many of the metabolic and non-metabolic diseases in the body, and as a strong affective factor, after its establishment can influence the performance of other organs and systems. The aim of this study was to examine possible changes in the sensory threshold of pain in offspring born to mothers with metabolic alkalosis in comparison with those born to healthy mothers. methods: in this experimental study, 50 mature female Westar rats each weighing 180±20gr were randomly divided into five groups: the control left untreated, the sham received calcium carbonate solution for 30 days before and up to the end of pregnancy, and three experimental groups including: minimum (I), medium (II) and maximum (III) dose alkalosis. Sodium bicarbonate was dissolved in drinking water to produce alkalosis in experimental groups. These groups with minimum, medium and maximum alkalosis received 0. 2M Sodium bicarbonate for 10, 20 and 30 days before and up to the end of pregnancy respectively. Following birth of the offspring, the treatment was stopped, and rats used tap water to the end of the experiment. Offspring were kept to adulthood, and then, males and females were separated. The study of sensory threshold of pain was carried out on male offspring by formalin test procedure. Pain scores were recorded in acute and chronic stages and were compared among different groups. Data was statistically analyzed by SPSS software, and Tukey and Anova test. Findings: the results indicated a significant increase in the pain scores during acute(0-5min) and chronic stages(16-60min) of formalin test in male rat offspring born to mothers with metabolic alkalosis compared to control and sham groups (P 0.05) Conclusion: Metabolic alkalosis is one of the most important diseases that affect the nervous system and naturally, increases neuronal excitability. Thus, with such impacts, alkalosis could reduce the sensory threshold of pain in offspring born to mothers with metabolic alkalosis, thereby increases pain in them. In this way, it is possible to reduce the sensory threshold of pain in children by controlling metabolic alkalosis in pregnant mothers.