PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava

PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava

Abstract Background The phenylalanine ammonia lyase genes play crucial role in plant response to biotic and abiotic stresses. In this study, we characterized the role of PAL genes in increasing resistance to the Cassava brown streak virus that causes the economically important cassava brown streak d...

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Main Authors: Siji Kavil, Gerald Otti, Sophie Bouvaine, Andrew Armitage, Midatharahally N. Maruthi
Format: Article
Language:English
Published: BMC 2021-09-01
Series:Virology Journal
Subjects:
Cassava
CBSD
PAL1
Resistance
Induction
Online Access:https://doi.org/10.1186/s12985-021-01649-2
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spelling doaj-d46e10f097e44186b02a0a5fba2dc61e2021-09-12T11:03:04ZengBMCVirology Journal1743-422X2021-09-0118111010.1186/s12985-021-01649-2PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassavaSiji Kavil0Gerald Otti1Sophie Bouvaine2Andrew Armitage3Midatharahally N. Maruthi4Agriculture, Health and Environment Department, Natural Resources Institute, University of GreenwichAgriculture, Health and Environment Department, Natural Resources Institute, University of GreenwichAgriculture, Health and Environment Department, Natural Resources Institute, University of GreenwichAgriculture, Health and Environment Department, Natural Resources Institute, University of GreenwichAgriculture, Health and Environment Department, Natural Resources Institute, University of GreenwichAbstract Background The phenylalanine ammonia lyase genes play crucial role in plant response to biotic and abiotic stresses. In this study, we characterized the role of PAL genes in increasing resistance to the Cassava brown streak virus that causes the economically important cassava brown streak disease (CBSD) on cassava in Africa. Methods The whole transcriptomes of eight cassava varieties differing in resistance to CBSD were obtained at 1, 5 and 8 weeks after CBSV infection. Results Analysis of RNA-Seq data identified the overexpression of PAL1, PAL2, cinnamic acid and two chalcone synthase genes in CBSD-resistant cassava varieties, which was subsequently confirmed by RT-qPCR. The exogenous application of Acibenzolar-S-Methyl induced PAL1 gene expression to enhance resistance in the susceptible var. Kalawe. In contrast, the silencing of PAL1 by RNA interference led to increased susceptibility of the resistant var. Kaleso to CBSD. Conclusions PAL1 gene of the phenylpropanoid pathway has a major role in inducing resistance to CBSD in cassava plants and its early induction is key for CBSD resistance.https://doi.org/10.1186/s12985-021-01649-2CassavaCBSDPAL1ResistanceInduction
collection DOAJ
language English
format Article
sources DOAJ
author Siji Kavil
Gerald Otti
Sophie Bouvaine
Andrew Armitage
Midatharahally N. Maruthi
spellingShingle Siji Kavil
Gerald Otti
Sophie Bouvaine
Andrew Armitage
Midatharahally N. Maruthi
PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava
Virology Journal
Cassava
CBSD
PAL1
Resistance
Induction
author_facet Siji Kavil
Gerald Otti
Sophie Bouvaine
Andrew Armitage
Midatharahally N. Maruthi
author_sort Siji Kavil
title PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava
title_short PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava
title_full PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava
title_fullStr PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava
title_full_unstemmed PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava
title_sort pal1 gene of the phenylpropanoid pathway increases resistance to the cassava brown streak virus in cassava
publisher BMC
series Virology Journal
issn 1743-422X
publishDate 2021-09-01
description Abstract Background The phenylalanine ammonia lyase genes play crucial role in plant response to biotic and abiotic stresses. In this study, we characterized the role of PAL genes in increasing resistance to the Cassava brown streak virus that causes the economically important cassava brown streak disease (CBSD) on cassava in Africa. Methods The whole transcriptomes of eight cassava varieties differing in resistance to CBSD were obtained at 1, 5 and 8 weeks after CBSV infection. Results Analysis of RNA-Seq data identified the overexpression of PAL1, PAL2, cinnamic acid and two chalcone synthase genes in CBSD-resistant cassava varieties, which was subsequently confirmed by RT-qPCR. The exogenous application of Acibenzolar-S-Methyl induced PAL1 gene expression to enhance resistance in the susceptible var. Kalawe. In contrast, the silencing of PAL1 by RNA interference led to increased susceptibility of the resistant var. Kaleso to CBSD. Conclusions PAL1 gene of the phenylpropanoid pathway has a major role in inducing resistance to CBSD in cassava plants and its early induction is key for CBSD resistance.
topic Cassava
CBSD
PAL1
Resistance
Induction
url https://doi.org/10.1186/s12985-021-01649-2
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AT geraldotti pal1geneofthephenylpropanoidpathwayincreasesresistancetothecassavabrownstreakvirusincassava
AT sophiebouvaine pal1geneofthephenylpropanoidpathwayincreasesresistancetothecassavabrownstreakvirusincassava
AT andrewarmitage pal1geneofthephenylpropanoidpathwayincreasesresistancetothecassavabrownstreakvirusincassava
AT midatharahallynmaruthi pal1geneofthephenylpropanoidpathwayincreasesresistancetothecassavabrownstreakvirusincassava
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