Antibacterial properties of biologically formed chitosan nanoparticles using aqueous leaf extract of Ocimum basilicum

• Main Authors: Iman Rasaee, Maryam Ghannadnia, Hosein Honari
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2016-10-01
• Series: Nanomedicine Journal
• Subjects: Biosynthesis; Chitosan nanoparticles; Leaf extract; Ocimum basilicum
• Online Access: http://nmj.mums.ac.ir/article_7580_f16f80798e920b06aa0d57dbb05dbdc3.pdf

Objective(s): Chitosan nanoparticles (CNPs) were prepared based on the ionic gelation of chitoan with anionic compounds of Ocimum basilicum leaf extract. Materials and Methods: After addition of Ocimum basilicum leaf extract to chitosan solution, the physicochemical properties of the nanoparticles were determined by Field Emission Scanning Electron microscope (FESEM), Fourier Transform Infrared (FTIR) analysis, X-ray diffraction (XRD) Pattern, and Dynamic Light Scattering (DLS). The antibacterial activity of CNPs was evaluated by agar disc diffusion method. Results: The synthesized nanoparticles were found to be nearly spherical shape with size in the range of 135-729 nm. FTIR analysis revealed the presence of polyphenolic; proteins and alkaloids compounds act as effective agents for converting chitosan to CNPs. Moreover, the synthesized nanoparticles showed potent antibacterial activity against Gram positive and Gram negative bacteria. Conclusion: These results reveal that natural sources of materials such as plants could be used for preparation of CNPs instead of use of chemical substances.