A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses

A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses

Summary: We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infectio...

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Bibliographic Details
Main Authors: Touraj Aligholipour Farzani, Angela Chov, Alon Herschhorn
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Cell culture
Cell-based assays
Immunology
Molecular biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301969

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http://www.sciencedirect.com/science/article/pii/S2666166720301969

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