Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam Antibiotics

The aim of this study was to characterize the ultrastructural effects caused by β-lactam antibiotics in Klebsiella pneumoniae isolates. Three K. pneumoniae clinical isolates were selected for the study with resistance profiles for third-generation cephalosporins, aztreonam, and/or imipenem and with...

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Main Authors:	Dyana Leal Veras, Ana Catarina de Souza Lopes, Grasielle Vaz da Silva, Gabriel Gazzoni Araújo Gonçalves, Catarina Fernandes de Freitas, Fernanda Cristina Gomes de Lima, Maria Amélia Vieira Maciel, Ana Paula Sampaio Feitosa, Luiz Carlos Alves, Fábio André Brayner
Format:	Article
Language:	English
Published:	Hindawi Limited 2015-01-01
Series:	The Scientific World Journal
Online Access:	http://dx.doi.org/10.1155/2015/572128

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spelling	doaj-d43e13aa077a4f11a55bdb54807599882020-11-24T21:37:52ZengHindawi LimitedThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/572128572128Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam AntibioticsDyana Leal Veras0Ana Catarina de Souza Lopes1Grasielle Vaz da Silva2Gabriel Gazzoni Araújo Gonçalves3Catarina Fernandes de Freitas4Fernanda Cristina Gomes de Lima5Maria Amélia Vieira Maciel6Ana Paula Sampaio Feitosa7Luiz Carlos Alves8Fábio André Brayner9Setor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, BrazilDepartamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, BrazilSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, BrazilSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, BrazilDepartamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, BrazilDepartamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, BrazilDepartamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, BrazilSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, BrazilSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, BrazilSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, BrazilThe aim of this study was to characterize the ultrastructural effects caused by β-lactam antibiotics in Klebsiella pneumoniae isolates. Three K. pneumoniae clinical isolates were selected for the study with resistance profiles for third-generation cephalosporins, aztreonam, and/or imipenem and with different resistance genes for extended-spectrum β-lactamases (ESBL) or Klebsiella pneumoniae carbapenemase (KPC). Two K. pneumoniae isolates obtained from the microbiota, which were both resistant to amoxicillin and ampicillin, were also analyzed. In accordance with the susceptibility profile, the clinical isolates were subjected to subminimum inhibitory concentrations (sub-MICs) of cefotaxime, ceftazidime, aztreonam, and imipenem and the isolates from the microbiota to ampicillin and amoxicillin, for analysis by means of scanning and transmission electron microscopy. The K. pneumoniae isolates showed different morphological and ultrastructural changes after subjection to β-lactams tested at different concentrations, such as cell filamentation, loss of cytoplasmic material, and deformation of dividing septa. Our results demonstrate that K. pneumoniae isolates harboring different genes that encode for β-lactamases show cell alterations when subjected to different β-lactam antibiotics, thus suggesting that they possess residual activity in vitro, despite the phenotypic resistance presented in the isolates analyzed.http://dx.doi.org/10.1155/2015/572128
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Dyana Leal Veras Ana Catarina de Souza Lopes Grasielle Vaz da Silva Gabriel Gazzoni Araújo Gonçalves Catarina Fernandes de Freitas Fernanda Cristina Gomes de Lima Maria Amélia Vieira Maciel Ana Paula Sampaio Feitosa Luiz Carlos Alves Fábio André Brayner
spellingShingle	Dyana Leal Veras Ana Catarina de Souza Lopes Grasielle Vaz da Silva Gabriel Gazzoni Araújo Gonçalves Catarina Fernandes de Freitas Fernanda Cristina Gomes de Lima Maria Amélia Vieira Maciel Ana Paula Sampaio Feitosa Luiz Carlos Alves Fábio André Brayner Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam Antibiotics The Scientific World Journal
author_facet	Dyana Leal Veras Ana Catarina de Souza Lopes Grasielle Vaz da Silva Gabriel Gazzoni Araújo Gonçalves Catarina Fernandes de Freitas Fernanda Cristina Gomes de Lima Maria Amélia Vieira Maciel Ana Paula Sampaio Feitosa Luiz Carlos Alves Fábio André Brayner
author_sort	Dyana Leal Veras
title	Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam Antibiotics
title_short	Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam Antibiotics
title_full	Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam Antibiotics
title_fullStr	Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam Antibiotics
title_full_unstemmed	Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam Antibiotics
title_sort	ultrastructural changes in clinical and microbiota isolates of klebsiella pneumoniae carriers of genes blashv, blatem, blactx-m, or blakpc when subject to β-lactam antibiotics
publisher	Hindawi Limited
series	The Scientific World Journal
issn	2356-6140 1537-744X
publishDate	2015-01-01
description	The aim of this study was to characterize the ultrastructural effects caused by β-lactam antibiotics in Klebsiella pneumoniae isolates. Three K. pneumoniae clinical isolates were selected for the study with resistance profiles for third-generation cephalosporins, aztreonam, and/or imipenem and with different resistance genes for extended-spectrum β-lactamases (ESBL) or Klebsiella pneumoniae carbapenemase (KPC). Two K. pneumoniae isolates obtained from the microbiota, which were both resistant to amoxicillin and ampicillin, were also analyzed. In accordance with the susceptibility profile, the clinical isolates were subjected to subminimum inhibitory concentrations (sub-MICs) of cefotaxime, ceftazidime, aztreonam, and imipenem and the isolates from the microbiota to ampicillin and amoxicillin, for analysis by means of scanning and transmission electron microscopy. The K. pneumoniae isolates showed different morphological and ultrastructural changes after subjection to β-lactams tested at different concentrations, such as cell filamentation, loss of cytoplasmic material, and deformation of dividing septa. Our results demonstrate that K. pneumoniae isolates harboring different genes that encode for β-lactamases show cell alterations when subjected to different β-lactam antibiotics, thus suggesting that they possess residual activity in vitro, despite the phenotypic resistance presented in the isolates analyzed.
url	http://dx.doi.org/10.1155/2015/572128
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Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam Antibiotics

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