Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport Medium

The Hybrid Capture 2 (HC2) High-Risk HPV DNA assay serves as a triage test in the Slovenian national cervical cancer screening programme ZORA. To improve the limited analytical accuracy of HC2 test results near the cut-off value (1.0 relative light units/cut-off (RLU/CO)), we follow an internal prot...

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Main Authors:	Varl Jerneja, Ivanus Urska, Marinsek Ziva Pohar, Jerman Tine, Valencak Anja Ostrbenk, Poljak Mario, Prevodnik Veronika Kloboves
Format:	Article
Language:	English
Published:	Sciendo 2019-09-01
Series:	Radiology and Oncology
Subjects:	hybrid capture 2 hpv test borderline results grey zone specimen transport medium
Online Access:	https://doi.org/10.2478/raon-2019-0044

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spelling	doaj-d43bb42382974580a06f5a2d7209c35a2021-09-05T14:01:19ZengSciendoRadiology and Oncology1581-32072019-09-0153331632210.2478/raon-2019-0044raon-2019-0044Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport MediumVarl Jerneja0Ivanus Urska1Marinsek Ziva Pohar2Jerman Tine3Valencak Anja Ostrbenk4Poljak Mario5Prevodnik Veronika Kloboves6Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, SloveniaZORA National Cervical Cancer Screening Programme, Epidemiology and Cancer Registry, Institute of Oncology Ljubljana, Ljubljana, SloveniaDepartment of Cytopathology, Institute of Oncology Ljubljana, Ljubljana, SloveniaZORA National Cervical Cancer Screening Programme, Epidemiology and Cancer Registry, Institute of Oncology Ljubljana, Ljubljana, SloveniaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaDepartment of Cytopathology, Institute of Oncology Ljubljana, Ljubljana, SloveniaThe Hybrid Capture 2 (HC2) High-Risk HPV DNA assay serves as a triage test in the Slovenian national cervical cancer screening programme ZORA. To improve the limited analytical accuracy of HC2 test results near the cut-off value (1.0 relative light units/cut-off (RLU/CO)), we follow an internal protocol of repeating the test on all samples with borderline results within the 0.7-2.0 RLU/CO interval. The aim of the study was (i) to determine the clinical relevance of HC2 test results within three different “grey zones” for samples stored in Specimen Transport Medium (STM) and (ii) to determine whether the current algorithm of retesting “grey zone” STM specimens with the HC2 assay is clinically relevant.https://doi.org/10.2478/raon-2019-0044hybrid capture 2hpv testborderline resultsgrey zonespecimen transport medium
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Varl Jerneja Ivanus Urska Marinsek Ziva Pohar Jerman Tine Valencak Anja Ostrbenk Poljak Mario Prevodnik Veronika Kloboves
spellingShingle	Varl Jerneja Ivanus Urska Marinsek Ziva Pohar Jerman Tine Valencak Anja Ostrbenk Poljak Mario Prevodnik Veronika Kloboves Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport Medium Radiology and Oncology hybrid capture 2 hpv test borderline results grey zone specimen transport medium
author_facet	Varl Jerneja Ivanus Urska Marinsek Ziva Pohar Jerman Tine Valencak Anja Ostrbenk Poljak Mario Prevodnik Veronika Kloboves
author_sort	Varl Jerneja
title	Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport Medium
title_short	Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport Medium
title_full	Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport Medium
title_fullStr	Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport Medium
title_full_unstemmed	Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport Medium
title_sort	clinical relevance of the borderline results of the hybrid capture 2 high-risk hpv dna assay with cervical samples collected in specimen transport medium
publisher	Sciendo
series	Radiology and Oncology
issn	1581-3207
publishDate	2019-09-01
description	The Hybrid Capture 2 (HC2) High-Risk HPV DNA assay serves as a triage test in the Slovenian national cervical cancer screening programme ZORA. To improve the limited analytical accuracy of HC2 test results near the cut-off value (1.0 relative light units/cut-off (RLU/CO)), we follow an internal protocol of repeating the test on all samples with borderline results within the 0.7-2.0 RLU/CO interval. The aim of the study was (i) to determine the clinical relevance of HC2 test results within three different “grey zones” for samples stored in Specimen Transport Medium (STM) and (ii) to determine whether the current algorithm of retesting “grey zone” STM specimens with the HC2 assay is clinically relevant.
topic	hybrid capture 2 hpv test borderline results grey zone specimen transport medium
url	https://doi.org/10.2478/raon-2019-0044
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Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport Medium

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