The Droserasin 1 PSI: A Membrane-Interacting Antimicrobial Peptide from the Carnivorous Plant <i>Drosera capensis</i>

• Main Authors: Marc A. Sprague-Piercy, Jan C. Bierma, Marquise G. Crosby, Brooke P. Carpenter, Gemma R. Takahashi, Joana Paulino, Ivan Hung, Rongfu Zhang, John E. Kelly, Natalia Kozlyuk, Xi Chen, Carter T. Butts, Rachel W. Martin
• Format: Article
• Language: English
• Published: MDPI AG 2020-07-01
• Series: Biomolecules
• Subjects: antimicrobial peptide; membrane protein; lipid-protein interactions; solid-state NMR; <i>Drosera capensis</i>; carnivorous plant
• Online Access: https://www.mdpi.com/2218-273X/10/7/1069

The Droserasins, aspartic proteases from the carnivorous plant <i>Drosera capensis</i>, contain a 100-residue plant-specific insert (PSI) that is post-translationally cleaved and independently acts as an antimicrobial peptide. PSIs are of interest not only for their inhibition of microbial growth, but also because they modify the size of lipid vesicles and strongly interact with biological membranes. PSIs may therefore be useful for modulating lipid systems in NMR studies of membrane proteins. Here we present the expression and biophysical characterization of the Droserasin 1 PSI (D1 PSI.) This peptide is monomeric in solution and maintains its primarily <inline-formula> <math display="inline"> <semantics> <mi>α</mi> </semantics> </math> </inline-formula>-helical secondary structure over a wide range of temperatures and pH values, even under conditions where its three disulfide bonds are reduced. Vesicle fusion assays indicate that the D1 PSI strongly interacts with bacterial and fungal lipids at pH 5 and lower, consistent with the physiological pH of <i>D. capensis</i> mucilage. It binds lipids with a variety of head groups, highlighting its versatility as a potential stabilizer for lipid nanodiscs. Solid-state NMR spectra collected at a field strength of 36 T, using a unique series-connected hybrid magnet, indicate that the peptide is folded and strongly bound to the membrane. Molecular dynamics simulations indicate that the peptide is stable as either a monomer or a dimer in a lipid bilayer. Both the monomer and the dimer allow the passage of water through the membrane, albeit at different rates.