Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme

Abstract During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. Operational conditions are considered to be one of the main parameters responsible for such changes, especially when using the same me...

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Main Authors: Simona M. Miron, Ariane de Espindola, Patrick Dutournié, Arnaud Ponche
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-91564-x
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spelling doaj-d417bdbb17c847fc80a40bfa19f6045e2021-06-13T11:43:36ZengNature Publishing GroupScientific Reports2045-23222021-06-011111810.1038/s41598-021-91564-xStudy of the relationship between applied transmembrane pressure and antimicrobial activity of lysozymeSimona M. Miron0Ariane de Espindola1Patrick Dutournié2Arnaud Ponche3Institut de Science Des Matériaux de Mulhouse, Université de Haute-Alsace, CNRS IS2M UMR 7361Institut de Science Des Matériaux de Mulhouse, Université de Haute-Alsace, CNRS IS2M UMR 7361Institut de Science Des Matériaux de Mulhouse, Université de Haute-Alsace, CNRS IS2M UMR 7361Institut de Science Des Matériaux de Mulhouse, Université de Haute-Alsace, CNRS IS2M UMR 7361Abstract During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. Operational conditions are considered to be one of the main parameters responsible for such changes, especially when using the same membrane and molecule. The present study demonstrates that, the same cut-off membrane (commercial data) can result in different properties of the protein after filtration, due to their different pore network. The filtration of lysozyme, regardless of the membrane, produces a decrease in the membrane hydraulic permeability (between 10 and 30%) and an increase in its selectivity in terms of observed rejection rate (30%). For the filtrated lysozyme, it appears that the HPLC retention time increases depending on the membrane used. The antibacterial activity of the filtrated samples is lower than the native protein and decreases with the increase of the applied pressure reaching 55–60% loss for 12 bar which has not been reported in the literature before. The observed results by SEC-HPLC and bacteriological tests, suggest that the conformation of the filtrated molecules are indeed modified. These results highlight the relationship between protein conformation or activity and the imposed shear stress.https://doi.org/10.1038/s41598-021-91564-x
collection DOAJ
language English
format Article
sources DOAJ
author Simona M. Miron
Ariane de Espindola
Patrick Dutournié
Arnaud Ponche
spellingShingle Simona M. Miron
Ariane de Espindola
Patrick Dutournié
Arnaud Ponche
Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
Scientific Reports
author_facet Simona M. Miron
Ariane de Espindola
Patrick Dutournié
Arnaud Ponche
author_sort Simona M. Miron
title Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_short Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_full Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_fullStr Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_full_unstemmed Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_sort study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-06-01
description Abstract During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. Operational conditions are considered to be one of the main parameters responsible for such changes, especially when using the same membrane and molecule. The present study demonstrates that, the same cut-off membrane (commercial data) can result in different properties of the protein after filtration, due to their different pore network. The filtration of lysozyme, regardless of the membrane, produces a decrease in the membrane hydraulic permeability (between 10 and 30%) and an increase in its selectivity in terms of observed rejection rate (30%). For the filtrated lysozyme, it appears that the HPLC retention time increases depending on the membrane used. The antibacterial activity of the filtrated samples is lower than the native protein and decreases with the increase of the applied pressure reaching 55–60% loss for 12 bar which has not been reported in the literature before. The observed results by SEC-HPLC and bacteriological tests, suggest that the conformation of the filtrated molecules are indeed modified. These results highlight the relationship between protein conformation or activity and the imposed shear stress.
url https://doi.org/10.1038/s41598-021-91564-x
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