Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms

Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms

Summary: MNase-seq (micrococcal nuclease sequencing) is used to map nucleosome positions in eukaryotic genomes to study the relationship between chromatin structure and DNA-dependent processes. Current protocols require at least two days to isolate nucleosome-protected DNA fragments. We have develop...

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Bibliographic Details
Main Authors: Laura E. McKnight, Johnathan G. Crandall, Thomas B. Bailey, Orion G.B. Banks, Kona N. Orlandi, Vi N. Truong, Drake A. Donovan, Grace L. Waddell, Elizabeth T. Wiles, Scott D. Hansen, Eric U. Selker, Jeffrey N. McKnight
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Genomics
Sequencing
Model Organisms
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001933

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