Purification and characterization of an isoflavones conjugate hydrolyzing β-glucosidase (ICHG) from Cyamopsis tetragonoloba (guar)
A β-glucosidase with high specific activity towards isoflavone glycosidic conjugates was purified from seeds of Guar (Cyamopsis tetragonoloba) by ammonium sulphate precipitation followed by size exclusion and ion exchange chromatography. The pH and temperature optima of the purified Isoflavones conj...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2019-12-01
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Series: | Biochemistry and Biophysics Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580818303182 |