Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma

• Main Authors: Lorena Favaro Pavon, Luciana Cavalheiro Marti, Tatiana Tais Sibov, Liza Aya Mabuchi Miyaki, Suzana Maria Fleury Malheiros, Javier Bustamante Mamani, Reynaldo Andre Brandt, Guilherme Carvalhal Ribas, Jorge Roberto Pagura, Marcos Augusto Stavale Joaquim, Hallin Feres Junior, Lionel Fernel Gamarra
• Format: Article
• Language: English
• Published: Instituto Israelita de Ensino e Pesquisa Albert Einstein 2012-06-01
• Series: Einstein (São Paulo)
• Subjects: Glioblastoma; Cell culture; Neoplastic stem cells; Antigens
• Online Access: http://apps.einstein.br/revista/arquivos/PDF/2257-197-202.pdf

Objective: To establish the method of isolation and culture ofhuman glioblastoma neurospheres, and the purification of theirstem cells, followed by the process of obtaining tumor subspheres,immunophenotypically characterizing this clonogenic set. Methods:Through the processing of glioblastoma samples (n=3), the followingstrategy of action was adopted: (i) establish primary culture ofglioblastoma; (ii) isolation and culture of tumor neurospheres; (iii)purify cells that initiate tumors (CD133+) by magnetic separationsystem (MACS); (iv) obtain tumor subspheres; (v) study theexpression of the markers nestin, CD133, and GFAP. Results: Thestudy successfully described the process of isolation and culture ofglioblastoma subspheres, which consist of a number of clonogeniccells immunophenotypically characterized as neural, which areable to initiate tumor formation. Conclusion: These findings maycontribute to a better understanding of the process of gliomagenesis.