The Application of Pulsed Field Gel Electrophoresis in Clinical Studies

• Main Authors: Elaheh Gholami Parizad, Eskandar Gholami Parizad, Azar Valizadeh
• Format: Article
• Language: English
• Published: JCDR Research and Publications Private Limited 2016-01-01
• Series: Journal of Clinical and Diagnostic Research
• Subjects: clinical research tool; dna size; typing
• Online Access: https://jcdr.net/articles/PDF/7043/15718_CE[Ra]_F(Sh)_PF1(Vsu_Om)_PFA(AK)_PF2(PAG).pdf
• ISSN: 2249-782X; 0973-709X

Pulsed-field gel electrophoresis is a method applied in separating large segments of deoxyribonucleotide using an alternating and cross field. In a uniform magnetic field, components larger than 50kb pass a route through the gel and since the movement of DNA (Deoxyribonucleic acid) molecules are in a Zigzag form, separation of DNAs as bands carried out better via gel. PFGE in microbiology is a standard method which is used for typing of bacteria. It is also a very useful tool in epidemiological studies and gene mapping in microbes and mammalian cell, also motivated development of large-insert cloning system such as bacterial and yeast artifical chromosomes. In this method, close and similar species in terms of genetic patterns show alike profiles regarding DNA separation, and those ones which don’t have similarity or are less similar, reveal different separation profiles. So this feature can be used to determine the common species as the prevalence agent of a disease. PFGE can be utilized for monitoring and evaluating different micro-organisms in clinical samples and existing ones in soil and water. This method can also be a reliable and standard method in vaccine preparation. In recent decades, PFGE is highly regarded as a powerful tool in control, prevention and monitoring diseases in different populations.