Live cell imaging of single genomic loci with quantum dot-labeled TALEs

Live cell imaging of single genomic loci with quantum dot-labeled TALEs

Visualizing single genomic loci in living cells remains technically challenging. Here the authors adopt a strategy of labelling a pair of transcription activator-like effectors (TALEs) with differently coloured quantum dots and use them to identify integrated HIV-1 proviral DNA sequences in living c...

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Main Authors: Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Tianwei Tan, Xian-En Zhang, Zongqiang Cui
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms15318
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spelling doaj-d299f1264a2a475692ab68c7f85c28472021-05-11T07:42:55ZengNature Publishing GroupNature Communications2041-17232017-05-01811810.1038/ncomms15318Live cell imaging of single genomic loci with quantum dot-labeled TALEsYingxin Ma0Mingxiu Wang1Wei Li2Zhiping Zhang3Xiaowei Zhang4Tianwei Tan5Xian-En Zhang6Zongqiang Cui7State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesBeijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical TechnologyCAS Center for Biological Macromolecules, National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesVisualizing single genomic loci in living cells remains technically challenging. Here the authors adopt a strategy of labelling a pair of transcription activator-like effectors (TALEs) with differently coloured quantum dots and use them to identify integrated HIV-1 proviral DNA sequences in living cells.https://doi.org/10.1038/ncomms15318
collection DOAJ
language English
format Article
sources DOAJ
author Yingxin Ma
Mingxiu Wang
Wei Li
Zhiping Zhang
Xiaowei Zhang
Tianwei Tan
Xian-En Zhang
Zongqiang Cui
spellingShingle Yingxin Ma
Mingxiu Wang
Wei Li
Zhiping Zhang
Xiaowei Zhang
Tianwei Tan
Xian-En Zhang
Zongqiang Cui
Live cell imaging of single genomic loci with quantum dot-labeled TALEs
Nature Communications
author_facet Yingxin Ma
Mingxiu Wang
Wei Li
Zhiping Zhang
Xiaowei Zhang
Tianwei Tan
Xian-En Zhang
Zongqiang Cui
author_sort Yingxin Ma
title Live cell imaging of single genomic loci with quantum dot-labeled TALEs
title_short Live cell imaging of single genomic loci with quantum dot-labeled TALEs
title_full Live cell imaging of single genomic loci with quantum dot-labeled TALEs
title_fullStr Live cell imaging of single genomic loci with quantum dot-labeled TALEs
title_full_unstemmed Live cell imaging of single genomic loci with quantum dot-labeled TALEs
title_sort live cell imaging of single genomic loci with quantum dot-labeled tales
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2017-05-01
description Visualizing single genomic loci in living cells remains technically challenging. Here the authors adopt a strategy of labelling a pair of transcription activator-like effectors (TALEs) with differently coloured quantum dots and use them to identify integrated HIV-1 proviral DNA sequences in living cells.
url https://doi.org/10.1038/ncomms15318
work_keys_str_mv AT yingxinma livecellimagingofsinglegenomiclociwithquantumdotlabeledtales
AT mingxiuwang livecellimagingofsinglegenomiclociwithquantumdotlabeledtales
AT weili livecellimagingofsinglegenomiclociwithquantumdotlabeledtales
AT zhipingzhang livecellimagingofsinglegenomiclociwithquantumdotlabeledtales
AT xiaoweizhang livecellimagingofsinglegenomiclociwithquantumdotlabeledtales
AT tianweitan livecellimagingofsinglegenomiclociwithquantumdotlabeledtales
AT xianenzhang livecellimagingofsinglegenomiclociwithquantumdotlabeledtales
AT zongqiangcui livecellimagingofsinglegenomiclociwithquantumdotlabeledtales
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