Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method
Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody. Methods: The polyclonal antibody was produced against the BSA in rabbits. Then, the pure BSA was injec...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2016-12-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-651.pdf |
| Summary: | Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical
applications. The aim of this study was to purify bovine serum albumin (BSA)
using produced rabbit anti-BSA antibody.
Methods: The polyclonal antibody was produced against the BSA in rabbits. Then,
the pure BSA was injected to three white New Zealand rabbits. ELISA test was
done to evaluate antibody production. After antibody purification,the purified
antibody was attached to CNBr-activated sepharose and finally it was used for
purification of albumin from bovine serum. Western blotting analysis was used
for functional assessment of immunoaffinity purified BSA.
Results: The titer of anti-bovine albumin determined by ELISA was obtained 1:
256000. The SDS-PAGE showed up to 98% purity of isolated BSA and western
blotting confirmed the BSA functionality. Purified bovine serum albumin by
affinity chromatography showed a single band with molecular weight of 66 KDa.
Conclusion: Affinity chromatography using produced rabbit anti-BSA antibody would be
an economical and safe method for purification of BSA. |
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| ISSN: | 2228-5881 2251-7308 |