Quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]

Quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]

Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, re...

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Bibliographic Details
Main Authors: Goran Kungulovski, Albert Jeltsch
Format: Article
Language:English
Published: F1000 Research Ltd 2015-11-01
Series:F1000Research
Subjects:
Immune Response
Immunological Biomarkers
Online Access:http://f1000research.com/articles/4-1160/v2
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spelling doaj-d2503d017f7843d99ebd8f0f029454702020-11-25T03:12:08ZengF1000 Research LtdF1000Research2046-14022015-11-01410.12688/f1000research.7265.27991Quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]Goran Kungulovski0Albert Jeltsch1Institute of Biochemistry, Stuttgart University, Stuttgart, 70569, GermanyInstitute of Biochemistry, Stuttgart University, Stuttgart, 70569, GermanyHistone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would help to improve the reproducibility of experimental work in this field.http://f1000research.com/articles/4-1160/v2Immune ResponseImmunological Biomarkers
collection DOAJ
language English
format Article
sources DOAJ
author Goran Kungulovski
Albert Jeltsch
spellingShingle Goran Kungulovski
Albert Jeltsch
Quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]
F1000Research
Immune Response
Immunological Biomarkers
author_facet Goran Kungulovski
Albert Jeltsch
author_sort Goran Kungulovski
title Quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]
title_short Quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]
title_full Quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]
title_fullStr Quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]
title_full_unstemmed Quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]
title_sort quality of histone modification antibodies undermines chromatin biology research [version 2; referees: 1 approved, 2 approved with reservations]
publisher F1000 Research Ltd
series F1000Research
issn 2046-1402
publishDate 2015-11-01
description Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would help to improve the reproducibility of experimental work in this field.
topic Immune Response
Immunological Biomarkers
url http://f1000research.com/articles/4-1160/v2
work_keys_str_mv AT gorankungulovski qualityofhistonemodificationantibodiesundermineschromatinbiologyresearchversion2referees1approved2approvedwithreservations
AT albertjeltsch qualityofhistonemodificationantibodiesundermineschromatinbiologyresearchversion2referees1approved2approvedwithreservations
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