Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia

Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia

ABSTRACT A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) betwe...

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Main Authors: Diego H. Cáceres, Alejandra Zuluaga, Ángela M. Tabares, Tom Chiller, Ángel González, Beatriz L. Gómez
Format: Article
Language:English
Published: Universidade de São Paulo 2017-12-01
Series:Revista do Instituto de Medicina Tropical de São Paulo
Subjects:
Cryptococcosis
Cryptococcus
Antigen
Diagnosis
Point-of-care
Lateral Flow Assay
Immunochromatographic assay
Latex agglutination system
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000507&lng=en&tlng=en
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spelling doaj-d24b8ab85bdb4dd1957284864a131f062020-11-24T22:21:07ZengUniversidade de São PauloRevista do Instituto de Medicina Tropical de São Paulo1678-99462017-12-0159010.1590/s1678-9946201759076S0036-46652017005000507Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in ColombiaDiego H. CáceresAlejandra ZuluagaÁngela M. TabaresTom ChillerÁngel GonzálezBeatriz L. GómezABSTRACT A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS®) and CrAg® LFA was observed. CrAg® LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000507&lng=en&tlng=enCryptococcosisCryptococcusAntigenDiagnosisPoint-of-careLateral Flow AssayImmunochromatographic assayLatex agglutination system
collection DOAJ
language English
format Article
sources DOAJ
author Diego H. Cáceres
Alejandra Zuluaga
Ángela M. Tabares
Tom Chiller
Ángel González
Beatriz L. Gómez
spellingShingle Diego H. Cáceres
Alejandra Zuluaga
Ángela M. Tabares
Tom Chiller
Ángel González
Beatriz L. Gómez
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
Revista do Instituto de Medicina Tropical de São Paulo
Cryptococcosis
Cryptococcus
Antigen
Diagnosis
Point-of-care
Lateral Flow Assay
Immunochromatographic assay
Latex agglutination system
author_facet Diego H. Cáceres
Alejandra Zuluaga
Ángela M. Tabares
Tom Chiller
Ángel González
Beatriz L. Gómez
author_sort Diego H. Cáceres
title Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_short Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_full Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_fullStr Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_full_unstemmed Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_sort evaluation of a cryptococcal antigen lateral flow assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in colombia
publisher Universidade de São Paulo
series Revista do Instituto de Medicina Tropical de São Paulo
issn 1678-9946
publishDate 2017-12-01
description ABSTRACT A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS®) and CrAg® LFA was observed. CrAg® LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen.
topic Cryptococcosis
Cryptococcus
Antigen
Diagnosis
Point-of-care
Lateral Flow Assay
Immunochromatographic assay
Latex agglutination system
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000507&lng=en&tlng=en
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