| Summary: | The aim of this study was to characterize uterine, fecal, bedding, and airborne dust microbiota from postpartum dairy cows and their environment. The cows were managed by the free-stall housing system, and samples for microbiota and serum metabolite assessment were collected during summer and winter when the cows were at one and two months postpartum. Uterine microbiota varied between seasons; the five most prevalent taxa were <i>Enterobacteriaceae</i><i>, </i><i>Moraxellaceae</i><i>, </i><i>Ruminococcaceae</i><i>, </i><i>Staphylococcaceae</i>, and <i>Lactobacillaceae</i><i> </i>during summer, and <i>Ruminococcaceae</i><i>, </i><i>Lachnospiraceae</i><i>, </i><i>Bacteroidaceae</i><i>, </i><i>Moraxellaceae</i>, and <i>Clostridiaceae</i><i> </i>during winter. Although <i>Actinomycetaceae</i> and <i>Mycoplasmataceae</i><i> </i>were detected at high abundance in several uterine samples, the relationship between the uterine microbiota and serum metabolite concentrations was unclear. The fecal microbiota was stable regardless of the season, whereas bedding and airborne dust microbiota varied between summer and winter. With regards to uterine, bedding, and airborne dust microbiota, <i>Enterobacteriaceae</i><i>, </i><i>Moraxellaceae</i><i>, </i><i>Staphylococcaceae</i>, and <i>Lactobacillaceae</i> were more abundant during summer, and <i>Ruminococcaceae</i><i>, </i><i>Lachnospiraceae</i><i>, </i><i>Bacteroidaceae</i><i>, </i>and <i>Clostridiaceae</i> were more abundant during winter. Canonical analysis of principal coordinates confirmed the relationship between uterine and cowshed microbiota. These results indicated that the uterine microbiota may vary when the microbiota in cowshed environments changes.
|
|---|
