Electrophysiological Studies on The Dynamics of Luminance Adaptation in the Mouse Retina

To date, most studies involving in vivo electroretinography in mice are performed on steady state adapted animals. In this study, we focused on the dynamics of adaptation to high and low light levels in the mouse retina. Two flash electroretinogram (ERG) protocols and one flicker ERG protocol were e...

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Main Authors: Anneka Joachimsthaler, Tina I. Tsai, Jan Kremers
Format: Article
Language:English
Published: MDPI AG 2017-10-01
Series:Vision
Subjects:
ERG
Online Access:https://www.mdpi.com/2411-5150/1/4/23
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spelling doaj-d218971205e6445c802d458fe4160a272020-11-24T23:23:52ZengMDPI AGVision2411-51502017-10-01142310.3390/vision1040023vision1040023Electrophysiological Studies on The Dynamics of Luminance Adaptation in the Mouse RetinaAnneka Joachimsthaler0Tina I. Tsai1Jan Kremers2Department of Ophthamlology, University Hospital Erlangen, 91054 Erlangen, GermanyDepartment of Ophthamlology, University Hospital Erlangen, 91054 Erlangen, GermanyDepartment of Ophthamlology, University Hospital Erlangen, 91054 Erlangen, GermanyTo date, most studies involving in vivo electroretinography in mice are performed on steady state adapted animals. In this study, we focused on the dynamics of adaptation to high and low light levels in the mouse retina. Two flash electroretinogram (ERG) protocols and one flicker ERG protocol were employed. In the two flash ERG protocols, the animals were adapted to either 25 or 40 cd/m2 white light and ERGs were recorded for up to 15 min of adaptation. Afterwards, flash ERGs were recorded for up to 45 min of dark adaptation. Amplitudes of the flash ERG increased during light adaptation, while implicit times of the different wave components decreased. During subsequent dark adaptation, the amplitudes further increased. The increase in a-to-b-wave ratio indicated adaptational processes at the photoreceptor synapse. In the flicker ERG protocol, the responses to 12 Hz sinusoidal luminance modulation during the adaptation to 25 cd/m2 and a 1 cd/m2 mean luminances were recorded. The amplitudes of the first harmonic components in the flicker protocol decreased during light adaptation but increased during dark adaptation. This is at odds with the changes in the flash ERG, indicating that adaptation may be different in different retinal pathways.https://www.mdpi.com/2411-5150/1/4/23electrophysiologyERGmouseadaptation
collection DOAJ
language English
format Article
sources DOAJ
author Anneka Joachimsthaler
Tina I. Tsai
Jan Kremers
spellingShingle Anneka Joachimsthaler
Tina I. Tsai
Jan Kremers
Electrophysiological Studies on The Dynamics of Luminance Adaptation in the Mouse Retina
Vision
electrophysiology
ERG
mouse
adaptation
author_facet Anneka Joachimsthaler
Tina I. Tsai
Jan Kremers
author_sort Anneka Joachimsthaler
title Electrophysiological Studies on The Dynamics of Luminance Adaptation in the Mouse Retina
title_short Electrophysiological Studies on The Dynamics of Luminance Adaptation in the Mouse Retina
title_full Electrophysiological Studies on The Dynamics of Luminance Adaptation in the Mouse Retina
title_fullStr Electrophysiological Studies on The Dynamics of Luminance Adaptation in the Mouse Retina
title_full_unstemmed Electrophysiological Studies on The Dynamics of Luminance Adaptation in the Mouse Retina
title_sort electrophysiological studies on the dynamics of luminance adaptation in the mouse retina
publisher MDPI AG
series Vision
issn 2411-5150
publishDate 2017-10-01
description To date, most studies involving in vivo electroretinography in mice are performed on steady state adapted animals. In this study, we focused on the dynamics of adaptation to high and low light levels in the mouse retina. Two flash electroretinogram (ERG) protocols and one flicker ERG protocol were employed. In the two flash ERG protocols, the animals were adapted to either 25 or 40 cd/m2 white light and ERGs were recorded for up to 15 min of adaptation. Afterwards, flash ERGs were recorded for up to 45 min of dark adaptation. Amplitudes of the flash ERG increased during light adaptation, while implicit times of the different wave components decreased. During subsequent dark adaptation, the amplitudes further increased. The increase in a-to-b-wave ratio indicated adaptational processes at the photoreceptor synapse. In the flicker ERG protocol, the responses to 12 Hz sinusoidal luminance modulation during the adaptation to 25 cd/m2 and a 1 cd/m2 mean luminances were recorded. The amplitudes of the first harmonic components in the flicker protocol decreased during light adaptation but increased during dark adaptation. This is at odds with the changes in the flash ERG, indicating that adaptation may be different in different retinal pathways.
topic electrophysiology
ERG
mouse
adaptation
url https://www.mdpi.com/2411-5150/1/4/23
work_keys_str_mv AT annekajoachimsthaler electrophysiologicalstudiesonthedynamicsofluminanceadaptationinthemouseretina
AT tinaitsai electrophysiologicalstudiesonthedynamicsofluminanceadaptationinthemouseretina
AT jankremers electrophysiologicalstudiesonthedynamicsofluminanceadaptationinthemouseretina
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