Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro

Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro

Backbone extended monomers are poorly compatible with the natural ribosomes, impeding their polymerization into polypeptides. Here the authors design non-canonical amino acid analogs with cyclic structures or extended carbon chains and used an engineered ribosome to improve tRNA-charging and incorpo...

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Main Authors: Joongoo Lee, Kevin J. Schwarz, Do Soon Kim, Jeffrey S. Moore, Michael C. Jewett
Format: Article
Language:English
Published: Nature Publishing Group 2020-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-18001-x
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spelling doaj-d1f0310e1e4a4c63ab01552ba5fb1c772021-08-29T11:38:41ZengNature Publishing GroupNature Communications2041-17232020-08-011111810.1038/s41467-020-18001-xRibosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitroJoongoo Lee0Kevin J. Schwarz1Do Soon Kim2Jeffrey S. Moore3Michael C. Jewett4Department of Chemical and Biological Engineering and Center for Synthetic Biology, Northwestern UniversityDepartment of Chemistry, University of Illinois at Urbana-ChampaignDepartment of Chemical and Biological Engineering and Center for Synthetic Biology, Northwestern UniversityDepartment of Chemistry, University of Illinois at Urbana-ChampaignDepartment of Chemical and Biological Engineering and Center for Synthetic Biology, Northwestern UniversityBackbone extended monomers are poorly compatible with the natural ribosomes, impeding their polymerization into polypeptides. Here the authors design non-canonical amino acid analogs with cyclic structures or extended carbon chains and used an engineered ribosome to improve tRNA-charging and incorporation into peptides.https://doi.org/10.1038/s41467-020-18001-x
collection DOAJ
language English
format Article
sources DOAJ
author Joongoo Lee
Kevin J. Schwarz
Do Soon Kim
Jeffrey S. Moore
Michael C. Jewett
spellingShingle Joongoo Lee
Kevin J. Schwarz
Do Soon Kim
Jeffrey S. Moore
Michael C. Jewett
Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro
Nature Communications
author_facet Joongoo Lee
Kevin J. Schwarz
Do Soon Kim
Jeffrey S. Moore
Michael C. Jewett
author_sort Joongoo Lee
title Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro
title_short Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro
title_full Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro
title_fullStr Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro
title_full_unstemmed Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro
title_sort ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-08-01
description Backbone extended monomers are poorly compatible with the natural ribosomes, impeding their polymerization into polypeptides. Here the authors design non-canonical amino acid analogs with cyclic structures or extended carbon chains and used an engineered ribosome to improve tRNA-charging and incorporation into peptides.
url https://doi.org/10.1038/s41467-020-18001-x
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AT kevinjschwarz ribosomemediatedpolymerizationoflongchaincarbonandcyclicaminoacidsintopeptidesinvitro
AT dosoonkim ribosomemediatedpolymerizationoflongchaincarbonandcyclicaminoacidsintopeptidesinvitro
AT jeffreysmoore ribosomemediatedpolymerizationoflongchaincarbonandcyclicaminoacidsintopeptidesinvitro
AT michaelcjewett ribosomemediatedpolymerizationoflongchaincarbonandcyclicaminoacidsintopeptidesinvitro
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