Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish

Summary: Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes produced by the ribosome pause and thus allows...

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Bibliographic Details
Main Authors: Mari Mito, Yuichiro Mishima, Shintaro Iwasaki
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301556