Aquaporin 3 promotes human extravillous trophoblast migration and invasion

Abstract Problem Does aquaporin 3 (AQP3) affect the migration and invasion of human extravillous trophoblast (HTR8/Svneo) cells? Method of study A lentivirus infection system was used to construct stable cell lines with either AQP3 knockdown or overexpression. RT-PCR and western blotting were used t...

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Main Authors: Yingqi Nong, Shifen Li, Wenjuan Liu, Xiqian Zhang, Lin Fan, Ye Chen, Qianwen Huang, Qianyu Zhang, Fenghua Liu
Format: Article
Language:English
Published: BMC 2021-03-01
Series:Reproductive Biology and Endocrinology
Subjects:
Online Access:https://doi.org/10.1186/s12958-021-00726-z
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spelling doaj-d1abb235e4084e8abd20e690b2ef68dd2021-04-04T11:10:42ZengBMCReproductive Biology and Endocrinology1477-78272021-03-0119111110.1186/s12958-021-00726-zAquaporin 3 promotes human extravillous trophoblast migration and invasionYingqi Nong0Shifen Li1Wenjuan Liu2Xiqian Zhang3Lin Fan4Ye Chen5Qianwen Huang6Qianyu Zhang7Fenghua Liu8The First Affiliated Hospital of Jinan UniversityReproductive Medicine Center, Affiliated Shenzhen City Maternity and Child Healthcare Hospital of Southern Medical UniversityDepartment of Reproductive Health and Infertility, Guangdong Women and Children HospitalDepartment of Reproductive Health and Infertility, Guangdong Women and Children HospitalDepartment of Reproductive Health and Infertility, Guangdong Women and Children HospitalDepartment of Reproductive Health and Infertility, Guangdong Women and Children HospitalDepartment of Reproductive Health and Infertility, Guangdong Women and Children HospitalDepartment of Reproductive Health and Infertility, Guangdong Women and Children HospitalDepartment of Reproductive Health and Infertility, Guangdong Women and Children HospitalAbstract Problem Does aquaporin 3 (AQP3) affect the migration and invasion of human extravillous trophoblast (HTR8/Svneo) cells? Method of study A lentivirus infection system was used to construct stable cell lines with either AQP3 knockdown or overexpression. RT-PCR and western blotting were used to verify the efficiencies of AQP3 knockdown or overexpression in HTR8/Svneo cells at mRNA and protein levels, respectively. Cell Counting Kit-8 and flow cytometry assays were used to detect the influence of AQP3 knockdown or overexpression on proliferation and apoptosis of HTR8/Svneo cells. In addition, wound healing and Transwell invasion assays were used to detect the effects of AQP3 knockdown or overexpression on migration and invasion capabilities of HTR8/Svneo cells. An Agilent gene chip was used to screen for significant differentially expressed genes after AQP3 knockdown. Finally, mechanisms by which AQP3 influences the migration and invasion of HTR8/Svneo cells were explored using bioinformatic analysis. Results Compared with controls, migration and invasion capabilities of HTR8/Svneo cells were significantly reduced after AQP3 knockdown, and significantly increased after AQP3 overexpression. Subsequent bioinformatic analysis of gene chip expression profiles indicated downregulation of genes related to adhesion such as PDGF-B, as well as signaling pathways (such as PIK3/AKT, NF-κB, and TNF) after AQP3 knockdown. Conclusions AQP3 could significantly promote migration and invasion capabilities of human extravillous trophoblasts, it may mediate embryo invasion and adhesion to endometrium by regulating PDGF-B, PIK3/AKT signaling pathways, although this requires further verification.https://doi.org/10.1186/s12958-021-00726-zAquaporin 3 (AQP3)Embryo implantationHuman extravillous trophoblastMigrationInvasion
collection DOAJ
language English
format Article
sources DOAJ
author Yingqi Nong
Shifen Li
Wenjuan Liu
Xiqian Zhang
Lin Fan
Ye Chen
Qianwen Huang
Qianyu Zhang
Fenghua Liu
spellingShingle Yingqi Nong
Shifen Li
Wenjuan Liu
Xiqian Zhang
Lin Fan
Ye Chen
Qianwen Huang
Qianyu Zhang
Fenghua Liu
Aquaporin 3 promotes human extravillous trophoblast migration and invasion
Reproductive Biology and Endocrinology
Aquaporin 3 (AQP3)
Embryo implantation
Human extravillous trophoblast
Migration
Invasion
author_facet Yingqi Nong
Shifen Li
Wenjuan Liu
Xiqian Zhang
Lin Fan
Ye Chen
Qianwen Huang
Qianyu Zhang
Fenghua Liu
author_sort Yingqi Nong
title Aquaporin 3 promotes human extravillous trophoblast migration and invasion
title_short Aquaporin 3 promotes human extravillous trophoblast migration and invasion
title_full Aquaporin 3 promotes human extravillous trophoblast migration and invasion
title_fullStr Aquaporin 3 promotes human extravillous trophoblast migration and invasion
title_full_unstemmed Aquaporin 3 promotes human extravillous trophoblast migration and invasion
title_sort aquaporin 3 promotes human extravillous trophoblast migration and invasion
publisher BMC
series Reproductive Biology and Endocrinology
issn 1477-7827
publishDate 2021-03-01
description Abstract Problem Does aquaporin 3 (AQP3) affect the migration and invasion of human extravillous trophoblast (HTR8/Svneo) cells? Method of study A lentivirus infection system was used to construct stable cell lines with either AQP3 knockdown or overexpression. RT-PCR and western blotting were used to verify the efficiencies of AQP3 knockdown or overexpression in HTR8/Svneo cells at mRNA and protein levels, respectively. Cell Counting Kit-8 and flow cytometry assays were used to detect the influence of AQP3 knockdown or overexpression on proliferation and apoptosis of HTR8/Svneo cells. In addition, wound healing and Transwell invasion assays were used to detect the effects of AQP3 knockdown or overexpression on migration and invasion capabilities of HTR8/Svneo cells. An Agilent gene chip was used to screen for significant differentially expressed genes after AQP3 knockdown. Finally, mechanisms by which AQP3 influences the migration and invasion of HTR8/Svneo cells were explored using bioinformatic analysis. Results Compared with controls, migration and invasion capabilities of HTR8/Svneo cells were significantly reduced after AQP3 knockdown, and significantly increased after AQP3 overexpression. Subsequent bioinformatic analysis of gene chip expression profiles indicated downregulation of genes related to adhesion such as PDGF-B, as well as signaling pathways (such as PIK3/AKT, NF-κB, and TNF) after AQP3 knockdown. Conclusions AQP3 could significantly promote migration and invasion capabilities of human extravillous trophoblasts, it may mediate embryo invasion and adhesion to endometrium by regulating PDGF-B, PIK3/AKT signaling pathways, although this requires further verification.
topic Aquaporin 3 (AQP3)
Embryo implantation
Human extravillous trophoblast
Migration
Invasion
url https://doi.org/10.1186/s12958-021-00726-z
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