Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds

Invertase, the key enzyme responsible for sucrose hydrolysis. Inhibition of invertase can decrease the postprandial blood sugar level in diabetic patients and keep the blood glucose level normal where cytotoxicity to fast-growing cells like those of brine shrimp (Artemia salina) nauplii is a great...

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Main Authors: Asim Debnath, Mohammad Salim, Md Faruque Miah, Md Rezaul Karim, Md Jahangir Alam
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2020-06-01
Series:Traditional and Integrative Medicine
Subjects:
Online Access:https://jtim.tums.ac.ir/index.php/jtim/article/view/232
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spelling doaj-d1a512c265654ebaa89fc4892c6e26602020-12-02T05:47:29ZengTehran University of Medical SciencesTraditional and Integrative Medicine2476-51042476-51122020-06-015210.18502/tim.v5i2.3626Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum SeedsAsim Debnath0Mohammad Salim1Md Faruque Miah2Md Rezaul Karim3Md Jahangir Alam4Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, BangladeshDepartment of Chemistry, Shahjalal University of Science and Technology, Sylhet-3114, BangladeshDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, BangladeshBIRDEM General Hospital, Shahbagh Square, Dhaka-1000, Bangladesh1Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh Invertase, the key enzyme responsible for sucrose hydrolysis. Inhibition of invertase can decrease the postprandial blood sugar level in diabetic patients and keep the blood glucose level normal where cytotoxicity to fast-growing cells like those of brine shrimp (Artemia salina) nauplii is a great measurement for further important drug development like anticancer drugs. This study was aimed to investigate potential anti-diabetic and cytotoxic activities of the ethanol and acetone extracts of Swietenia macrophylla leaves, Syzygium cumini and Trigonella foenum-graecum seeds. Invertase inhibition activities of S. macrophylla leaves, S. cumini, and T. foenum-graecum seeds were measured by spectrophotometrically using standard protocols and cytotoxicities were measured by brine shrimp lethality bioassay. Among the plant extracts, all ethanol extracts showed higher invertase inhibition activities than all acetone extracts. S. cumini seed ethanol extract showed the highest invertase inhibition activity whereas S. macrophylla leaves acetone extract showed the lowest invertase inhibition activity. The maximum toxicity was observed in ethanol extract of T. foenum-graecum seed whereas the lowest toxicity was observed in acetone extract of S. macrophylla leaves. Both ethanol and acetone extract of T. foenum-graecum seeds showed significant cytotoxic activities. This investigation suggested that S. cumini and T. foenum-graecum seeds possess potential antidiabetic activities and T. foenum-graecum seeds have potential cytotoxicity. https://jtim.tums.ac.ir/index.php/jtim/article/view/232Plant extractsAntidiabetic activityInvertase inhibition activitCytotoxicity
collection DOAJ
language English
format Article
sources DOAJ
author Asim Debnath
Mohammad Salim
Md Faruque Miah
Md Rezaul Karim
Md Jahangir Alam
spellingShingle Asim Debnath
Mohammad Salim
Md Faruque Miah
Md Rezaul Karim
Md Jahangir Alam
Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds
Traditional and Integrative Medicine
Plant extracts
Antidiabetic activity
Invertase inhibition activit
Cytotoxicity
author_facet Asim Debnath
Mohammad Salim
Md Faruque Miah
Md Rezaul Karim
Md Jahangir Alam
author_sort Asim Debnath
title Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds
title_short Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds
title_full Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds
title_fullStr Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds
title_full_unstemmed Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds
title_sort evaluation of invertase inhibition activities and cytotoxicity of ethanol and acetone extracts of swietenia macrophyllia leaves, syzygium cumini and trigonella foenum-graecum seeds
publisher Tehran University of Medical Sciences
series Traditional and Integrative Medicine
issn 2476-5104
2476-5112
publishDate 2020-06-01
description Invertase, the key enzyme responsible for sucrose hydrolysis. Inhibition of invertase can decrease the postprandial blood sugar level in diabetic patients and keep the blood glucose level normal where cytotoxicity to fast-growing cells like those of brine shrimp (Artemia salina) nauplii is a great measurement for further important drug development like anticancer drugs. This study was aimed to investigate potential anti-diabetic and cytotoxic activities of the ethanol and acetone extracts of Swietenia macrophylla leaves, Syzygium cumini and Trigonella foenum-graecum seeds. Invertase inhibition activities of S. macrophylla leaves, S. cumini, and T. foenum-graecum seeds were measured by spectrophotometrically using standard protocols and cytotoxicities were measured by brine shrimp lethality bioassay. Among the plant extracts, all ethanol extracts showed higher invertase inhibition activities than all acetone extracts. S. cumini seed ethanol extract showed the highest invertase inhibition activity whereas S. macrophylla leaves acetone extract showed the lowest invertase inhibition activity. The maximum toxicity was observed in ethanol extract of T. foenum-graecum seed whereas the lowest toxicity was observed in acetone extract of S. macrophylla leaves. Both ethanol and acetone extract of T. foenum-graecum seeds showed significant cytotoxic activities. This investigation suggested that S. cumini and T. foenum-graecum seeds possess potential antidiabetic activities and T. foenum-graecum seeds have potential cytotoxicity.
topic Plant extracts
Antidiabetic activity
Invertase inhibition activit
Cytotoxicity
url https://jtim.tums.ac.ir/index.php/jtim/article/view/232
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