Imaging small molecule-induced endosomal escape of siRNA

Imaging small molecule-induced endosomal escape of siRNA

Therapeutic siRNA becomes trapped in endosomes, limiting its efficacy. Here the authors use fluorescently-tagged galectin-9 as a biosensor for membrane damage to monitor endosomal escape of cholesterol-conjugated siRNA following treatment of small molecule membrane-destabilising drugs.

Bibliographic Details
Main Authors: Hampus Du Rietz, Hampus Hedlund, Sten Wilhelmson, Pontus Nordenfelt, Anders Wittrup
Format: Article
Language:English
Published: Nature Publishing Group 2020-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-15300-1
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spelling doaj-d19329c8d4634c19add07accb1157c0d2021-05-11T08:18:56ZengNature Publishing GroupNature Communications2041-17232020-04-0111111710.1038/s41467-020-15300-1Imaging small molecule-induced endosomal escape of siRNAHampus Du Rietz0Hampus Hedlund1Sten Wilhelmson2Pontus Nordenfelt3Anders Wittrup4Department of Clinical Sciences, Oncology, Faculty of Medicine, Lund UniversityDepartment of Clinical Sciences, Oncology, Faculty of Medicine, Lund UniversityDepartment of Clinical Sciences, Oncology, Faculty of Medicine, Lund UniversityDepartment of Clinical Sciences, Division of Infection Medicine, Faculty of Medicine, Lund UniversityDepartment of Clinical Sciences, Oncology, Faculty of Medicine, Lund UniversityTherapeutic siRNA becomes trapped in endosomes, limiting its efficacy. Here the authors use fluorescently-tagged galectin-9 as a biosensor for membrane damage to monitor endosomal escape of cholesterol-conjugated siRNA following treatment of small molecule membrane-destabilising drugs.https://doi.org/10.1038/s41467-020-15300-1
collection DOAJ
language English
format Article
sources DOAJ
author Hampus Du Rietz
Hampus Hedlund
Sten Wilhelmson
Pontus Nordenfelt
Anders Wittrup
spellingShingle Hampus Du Rietz
Hampus Hedlund
Sten Wilhelmson
Pontus Nordenfelt
Anders Wittrup
Imaging small molecule-induced endosomal escape of siRNA
Nature Communications
author_facet Hampus Du Rietz
Hampus Hedlund
Sten Wilhelmson
Pontus Nordenfelt
Anders Wittrup
author_sort Hampus Du Rietz
title Imaging small molecule-induced endosomal escape of siRNA
title_short Imaging small molecule-induced endosomal escape of siRNA
title_full Imaging small molecule-induced endosomal escape of siRNA
title_fullStr Imaging small molecule-induced endosomal escape of siRNA
title_full_unstemmed Imaging small molecule-induced endosomal escape of siRNA
title_sort imaging small molecule-induced endosomal escape of sirna
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-04-01
description Therapeutic siRNA becomes trapped in endosomes, limiting its efficacy. Here the authors use fluorescently-tagged galectin-9 as a biosensor for membrane damage to monitor endosomal escape of cholesterol-conjugated siRNA following treatment of small molecule membrane-destabilising drugs.
url https://doi.org/10.1038/s41467-020-15300-1
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AT hampushedlund imagingsmallmoleculeinducedendosomalescapeofsirna
AT stenwilhelmson imagingsmallmoleculeinducedendosomalescapeofsirna
AT pontusnordenfelt imagingsmallmoleculeinducedendosomalescapeofsirna
AT anderswittrup imagingsmallmoleculeinducedendosomalescapeofsirna
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