3D particle averaging and detection of macromolecular symmetry in localization microscopy

Adaptation of current algorithms to 3D SMLM data is currently problematic. Here the authors report a method that increases the signal-to-noise ratio and resolution of 3D single particle analysis in localization microscopy and enables determination of the symmetry groups of macromolecular complexes.

Bibliographic Details
Main Authors:	Hamidreza Heydarian, Maarten Joosten, Adrian Przybylski, Florian Schueder, Ralf Jungmann, Ben van Werkhoven, Jan Keller-Findeisen, Jonas Ries, Sjoerd Stallinga, Mark Bates, Bernd Rieger
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-05-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-22006-5

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spelling	doaj-d17ad19811dd4f2a9819d5862675ea692021-05-16T11:10:42ZengNature Publishing GroupNature Communications2041-17232021-05-011211910.1038/s41467-021-22006-53D particle averaging and detection of macromolecular symmetry in localization microscopyHamidreza Heydarian0Maarten Joosten1Adrian Przybylski2Florian Schueder3Ralf Jungmann4Ben van Werkhoven5Jan Keller-Findeisen6Jonas Ries7Sjoerd Stallinga8Mark Bates9Bernd Rieger10Department of Imaging Physics, Delft University of TechnologyDepartment of Imaging Physics, Delft University of TechnologyDepartment of NanoBiophotonics, Max Planck Institute for Biophysical ChemistryDepartment of Physics and Center for Nanoscience, Ludwig Maximilian UniversityDepartment of Physics and Center for Nanoscience, Ludwig Maximilian UniversityNetherlands eScience CenterDepartment of NanoBiophotonics, Max Planck Institute for Biophysical ChemistryCell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL)Department of Imaging Physics, Delft University of TechnologyDepartment of NanoBiophotonics, Max Planck Institute for Biophysical ChemistryDepartment of Imaging Physics, Delft University of TechnologyAdaptation of current algorithms to 3D SMLM data is currently problematic. Here the authors report a method that increases the signal-to-noise ratio and resolution of 3D single particle analysis in localization microscopy and enables determination of the symmetry groups of macromolecular complexes.https://doi.org/10.1038/s41467-021-22006-5
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Hamidreza Heydarian Maarten Joosten Adrian Przybylski Florian Schueder Ralf Jungmann Ben van Werkhoven Jan Keller-Findeisen Jonas Ries Sjoerd Stallinga Mark Bates Bernd Rieger
spellingShingle	Hamidreza Heydarian Maarten Joosten Adrian Przybylski Florian Schueder Ralf Jungmann Ben van Werkhoven Jan Keller-Findeisen Jonas Ries Sjoerd Stallinga Mark Bates Bernd Rieger 3D particle averaging and detection of macromolecular symmetry in localization microscopy Nature Communications
author_facet	Hamidreza Heydarian Maarten Joosten Adrian Przybylski Florian Schueder Ralf Jungmann Ben van Werkhoven Jan Keller-Findeisen Jonas Ries Sjoerd Stallinga Mark Bates Bernd Rieger
author_sort	Hamidreza Heydarian
title	3D particle averaging and detection of macromolecular symmetry in localization microscopy
title_short	3D particle averaging and detection of macromolecular symmetry in localization microscopy
title_full	3D particle averaging and detection of macromolecular symmetry in localization microscopy
title_fullStr	3D particle averaging and detection of macromolecular symmetry in localization microscopy
title_full_unstemmed	3D particle averaging and detection of macromolecular symmetry in localization microscopy
title_sort	3d particle averaging and detection of macromolecular symmetry in localization microscopy
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-05-01
description	Adaptation of current algorithms to 3D SMLM data is currently problematic. Here the authors report a method that increases the signal-to-noise ratio and resolution of 3D single particle analysis in localization microscopy and enables determination of the symmetry groups of macromolecular complexes.
url	https://doi.org/10.1038/s41467-021-22006-5
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3D particle averaging and detection of macromolecular symmetry in localization microscopy

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