| Summary: | 【Abstract】 Objective: To investigate the expres-sion of Caspase-3 and Hsp70 in rabbits after severe trau-matic brain injury (TBI) and to explore the feasibility of its
application in estimation of injury time in forensic medicine.
Methods: A rabbit model of heavy TBI was developed
by high velocity impact on the parietal bone with an iron
stick. Totally 8 healthy adult New Zealand white rabbits
were randomly divided into control group (n=2) and injury
group (n=6). Four hours after injury, tissue specimens from
the parietal lobe, temporal lobe, occipital lobe, cerebellum
and brainstem were harvested to detect the expression of
Hsp70 and Caspase-3 by immunohistochemistry. Besides,
the gray values of cells positive for Hsp70 and Caspase-3
were analyzed with an image analyzer.
Results: Immunohistochemistry staining demonstrated
a low level of Caspase-3 and Hsp70 expression in normal
control group. While in injury group, both the Caspase-3
and Hsp70 expression was significantly elevated (P<0.05).
Positive cells gathered around the lesion focus. Occipital
lobe and cerebellum had fewer positive cells while temporal
and brainstem had the fewest.
Conclusion: The expression of Caspase-3 and Hsp70
at an early stage following severe TBI is characteristic and
can be applied to estimate the time of injury.
Key words: Forensic sciences; Craniocerebral
trauma; Caspase-3; HSP70 Heat-shock proteins; Immu-nohistochemistry
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