Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites of fibrin molecule
Using the idea of “proline brackets” we have found four sites in fibrin amino acid sequence, and appropriate peptides were synthesized: γ69NPDESSKPN77, Bβ228QPDSSVKPY236, Bβ455RPFFPQ460 and Aα195LPSRDRQHLPL205. Turbidity and electron-microscopy analyses have demonstrated that synthetic peptide Аα195...
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National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2014-08-01
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| Series: | Ukrainian Biochemical Journal |
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| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Pozniak_4_14.pdf |
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doaj-d15ed199285b45c6b33a06309d2d2d402020-11-24T22:07:19ZengNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.Ukrainian Biochemical Journal2409-49432413-50032014-08-0186411912510.15407/ubj86.04.119Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites of fibrin moleculeT. A. Pozniak0 L. P. Urvant1 P. G. Gritsenko2 V. I. Chernishov3N. A. Pydiura4 E. V. Lugovskoi5 S. V. Komisarenko6Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Using the idea of “proline brackets” we have found four sites in fibrin amino acid sequence, and appropriate peptides were synthesized: γ69NPDESSKPN77, Bβ228QPDSSVKPY236, Bβ455RPFFPQ460 and Aα195LPSRDRQHLPL205. Turbidity and electron-microscopy analyses have demonstrated that synthetic peptide Аα195-205 specifically inhibited the stage of fibrin protofibril formation and peptide γ69-77 – the stage of fibrin protofibril lateral association. The data obtained testify that there are the sites involved in these processes in the appropriate amino acid sequences of fibrin molecule.http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Pozniak_4_14.pdfinhibitorspeptidesproline bracketssites of fibrin polymerization |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
T. A. Pozniak L. P. Urvant P. G. Gritsenko V. I. Chernishov N. A. Pydiura E. V. Lugovskoi S. V. Komisarenko |
| spellingShingle |
T. A. Pozniak L. P. Urvant P. G. Gritsenko V. I. Chernishov N. A. Pydiura E. V. Lugovskoi S. V. Komisarenko Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites of fibrin molecule Ukrainian Biochemical Journal inhibitors peptides proline brackets sites of fibrin polymerization |
| author_facet |
T. A. Pozniak L. P. Urvant P. G. Gritsenko V. I. Chernishov N. A. Pydiura E. V. Lugovskoi S. V. Komisarenko |
| author_sort |
T. A. Pozniak |
| title |
Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites of fibrin molecule |
| title_short |
Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites of fibrin molecule |
| title_full |
Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites of fibrin molecule |
| title_fullStr |
Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites of fibrin molecule |
| title_full_unstemmed |
Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites of fibrin molecule |
| title_sort |
inhibition of fibrin polymerization by synthetic peptides corresponding to аα195-205 and γ69-77 sites of fibrin molecule |
| publisher |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. |
| series |
Ukrainian Biochemical Journal |
| issn |
2409-4943 2413-5003 |
| publishDate |
2014-08-01 |
| description |
Using the idea of “proline brackets” we have found four sites in fibrin amino acid sequence, and appropriate peptides were synthesized: γ69NPDESSKPN77, Bβ228QPDSSVKPY236, Bβ455RPFFPQ460 and Aα195LPSRDRQHLPL205. Turbidity and electron-microscopy analyses have demonstrated that synthetic peptide Аα195-205 specifically inhibited the stage of fibrin protofibril formation and peptide γ69-77 – the stage of fibrin protofibril lateral association. The data obtained testify that there are the sites involved in these processes in the appropriate amino acid sequences of fibrin molecule. |
| topic |
inhibitors peptides proline brackets sites of fibrin polymerization |
| url |
http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Pozniak_4_14.pdf |
| work_keys_str_mv |
AT tapozniak inhibitionoffibrinpolymerizationbysyntheticpeptidescorrespondingtoaa195205andg6977sitesoffibrinmolecule AT lpurvant inhibitionoffibrinpolymerizationbysyntheticpeptidescorrespondingtoaa195205andg6977sitesoffibrinmolecule AT pggritsenko inhibitionoffibrinpolymerizationbysyntheticpeptidescorrespondingtoaa195205andg6977sitesoffibrinmolecule AT vichernishov inhibitionoffibrinpolymerizationbysyntheticpeptidescorrespondingtoaa195205andg6977sitesoffibrinmolecule AT napydiura inhibitionoffibrinpolymerizationbysyntheticpeptidescorrespondingtoaa195205andg6977sitesoffibrinmolecule AT evlugovskoi inhibitionoffibrinpolymerizationbysyntheticpeptidescorrespondingtoaa195205andg6977sitesoffibrinmolecule AT svkomisarenko inhibitionoffibrinpolymerizationbysyntheticpeptidescorrespondingtoaa195205andg6977sitesoffibrinmolecule |
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1725821021892640768 |