Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites of fibrin molecule

• Main Authors: T. A. Pozniak, L. P. Urvant, P. G. Gritsenko, V. I. Chernishov, N. A. Pydiura, E. V. Lugovskoi, S. V. Komisarenko
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2014-08-01
• Series: Ukrainian Biochemical Journal
• Subjects: inhibitors; peptides; proline brackets; sites of fibrin polymerization
• Online Access: http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Pozniak_4_14.pdf
• ISSN: 2409-4943; 2413-5003

Using the idea of “proline brackets” we have found four sites in fibrin amino acid sequence, and appropriate peptides were synthesized: γ69NPDESSKPN77, Bβ228QPDSSVKPY236, Bβ455RPFFPQ460 and Aα195LPSRDRQHLPL205. Turbidity and electron-microscopy analyses have demonstrated that synthetic peptide Аα195-205 specifically inhibited the stage of fibrin protofibril formation and peptide γ69-77 – the stage of fibrin protofibril lateral association. The data obtained testify that there are the sites involved in these processes in the appropriate amino acid sequences of fibrin molecule.