Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing

Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing

Summary: Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer meta...

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Bibliographic Details
Main Authors: Kei Takahashi, Shimpei I. Kubota, Shogo Ehata, Hiroki R. Ueda, Kohei Miyazono
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Cancer
Microscopy
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301787
Description
Summary:Summary: Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry.For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).
ISSN:2666-1667

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