Non-Viable Lactobacillus Casei Beneficially Modulates Poly I:C Immune Response in Co-Cultures of Human Cells

• Main Authors: Elisa Vintiñi, Marcela Medina
• Format: Article
• Language: English
• Published: Shiraz University of Medical Sciences 2017-12-01
• Series: Iranian Journal of Immunology
• Subjects: A549 Cell; Immunomodulation; non-viable Lactobacillus; poly I:C; PBMC
• Online Access: http://iji.sums.ac.ir/article_39328_f12863523edece69a6bfabf6e8c2692e.pdf
• ISSN: 1735-1383; 1735-367X

<strong> </strong><br /> <strong>Background:</strong> Polyinosinic:polycytidylic acid (Poly-IC) has been used as a viral stimulus to mimic <em>in vivo</em> and <em>in vitro</em> infection induced by some viruses. <strong>Objective:</strong> To determine whether non-viable <em>Lactobacillus casei</em> CRL431 (LcM) can modulate the immune response induced by Poly I:C in co-culture models of peripheral blood mononuclear cells (PBMC) and A549 cells. <strong>Methods:</strong> T and NK cell activation was evaluated by flow cytometry and levels of TNF-α, IFN-γ, IL-10, IL-29, and IL-17 by ELISA. Cells in direct contact with A549 (PBMC-A549) and cells with no contact with it (PBMC//A549) were used for this purpose. PBMCs alone and both co-culture systems were stimulated for 24 h with the following stimuli: LPS (10 µg/ml), LcM (10⁶ UFC/ml), Poly I:C (2 µg/ml), Poly I:C+LcM, and LcM (3 h)+Poly I:C. Moreover, unstimulated cells were used as a control. <strong>Results:</strong> Poly I:C and LcM (3 h)+Poly I:C in PBMC-A549 showed a significant increase in the percentage of CD8+ expression (p<0.05). All stimuli induced significant activation from T CD4+, CD8+ cells compared with unstimulated PBMCs in both co-culture cells system. However, activation percentages were higher in direct co-culture. Poly I:C induced a higher level of pro-inflammatory TNF-α and IFN-γ cytokines as well as IL-17 and IL-29 with lower IL-10 levels in both co-culture systems while LcM induced a beneficial pattern of cytokines that would regulate Poly I:C effect. <strong>Conclusion:</strong> This <em>in vitro</em> model allowed us to highlight the potential of LcM as a modulator of anti-viral immune response and suggest its potential use in formulations against RNA respiratory viruses.