Quantitative assay to detect bacterial glycan-degrading enzyme activities in mouse and human fecal samples

Summary: The gut microbiome expresses a multitude of enzymes degrading polysaccharides in dietary plant fibers and in host-secreted mucus. The quantitative detection of these glycan-degrading enzymes in fecal samples is important to elucidate the functional activity of the microbiome in health and d...

Full description

Bibliographic Details
Main Authors: Alex Steimle, Erica T. Grant, Mahesh S. Desai
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000332