Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation.

Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation.

Parkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, i...

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Main Authors: Lynn Burchell, Viduth K Chaugule, Helen Walden
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3319606?pdf=render
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spelling doaj-d0daaec419f54ab891600efb7b7a9df22020-11-25T01:38:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3474810.1371/journal.pone.0034748Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation.Lynn BurchellViduth K ChauguleHelen WaldenParkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, influence the physical stability and activity of Parkin in and out of cells, perturbing the autoinhibited native state of Parkin, resulting in an active-for-autoubiquitination species.http://europepmc.org/articles/PMC3319606?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Lynn Burchell
Viduth K Chaugule
Helen Walden
spellingShingle Lynn Burchell
Viduth K Chaugule
Helen Walden
Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation.
PLoS ONE
author_facet Lynn Burchell
Viduth K Chaugule
Helen Walden
author_sort Lynn Burchell
title Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation.
title_short Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation.
title_full Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation.
title_fullStr Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation.
title_full_unstemmed Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation.
title_sort small, n-terminal tags activate parkin e3 ubiquitin ligase activity by disrupting its autoinhibited conformation.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description Parkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, influence the physical stability and activity of Parkin in and out of cells, perturbing the autoinhibited native state of Parkin, resulting in an active-for-autoubiquitination species.
url http://europepmc.org/articles/PMC3319606?pdf=render
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AT viduthkchaugule smallnterminaltagsactivateparkine3ubiquitinligaseactivitybydisruptingitsautoinhibitedconformation
AT helenwalden smallnterminaltagsactivateparkine3ubiquitinligaseactivitybydisruptingitsautoinhibitedconformation
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