Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria

Objective: The spread of carbapenemase-producing Enterobacteriaceae is a global challenge that leads to an increase in health-care cost, treatment failures, high morbidity, and mortality. This study was aimed at determining the prevalence of New-Delhi Metallo Beta-Lactamase (NDM), and oxacillinase-4...

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Main Authors: Ijeoma Maryrose Ajuba, Comfort Nne Akujobi, Iloduba Nnaemeka Aghanya, Simon Nkpeh Ushie, Akachukwu Egwu Okoro, Robinson Ogochukwu Ofiaeli, Chibuike Jesse Ezeama, Michel Chiedu Egbuniwe, Mabel Ogochukwu Okeke
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2020-01-01
Series:Nigerian Journal of Medicine
Subjects:
Online Access:http://www.njmonline.org/article.asp?issn=1115-2613;year=2020;volume=29;issue=4;spage=701;epage=706;aulast=Ajuba
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spelling doaj-d0d2cc21b92946a1934c98ff32d441c62021-08-09T10:22:56ZengWolters Kluwer Medknow PublicationsNigerian Journal of Medicine1115-26132020-01-0129470170610.4103/NJM.NJM_165_20Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern NigeriaIjeoma Maryrose AjubaComfort Nne AkujobiIloduba Nnaemeka AghanyaSimon Nkpeh UshieAkachukwu Egwu OkoroRobinson Ogochukwu OfiaeliChibuike Jesse EzeamaMichel Chiedu EgbuniweMabel Ogochukwu OkekeObjective: The spread of carbapenemase-producing Enterobacteriaceae is a global challenge that leads to an increase in health-care cost, treatment failures, high morbidity, and mortality. This study was aimed at determining the prevalence of New-Delhi Metallo Beta-Lactamase (NDM), and oxacillinase-48 (OXA-48) genes in clinical isolates of Escherichia coli obtained from a tertiary hospital in Nnewi, South-eastern, Nigeria. Materials and Methods: E. coli isolated from several clinical specimens including blood, urine, and wound swabs from patients receiving care at the hospital, were screened for resistance to meropenem and ertapenem antibiotics (Oxoid, UK) by the Kirby–Bauer disk-diffusion method. All isolates, which showed reduced sensitivity to the tested antibiotics, were then subjected to phenotypic confirmation of carbapenemase production using the Modified Hodge test. The NDM and OXA-48 genes were then detected using the polymerase chain reaction techniques. Results: Of the 187 E. coli isolates, 41 (21.9%) screened positive as suspected carbapenemase producers, while the prevalence of carbapenemase-producing E. coli in this study was 21/187 (11.23%). The prevalence of NDM and OXA-48 genes in the entire sample population was 3/187 (1.6%) and 12/187 (6.4%), respectively. Conclusion: The results obtained showed that NDM and OXA-48 carbapenemase-mediated resistance occurred in the study location. Hence, a reinforcement of infection prevention and control practices in the hospital will be required to curb the propagation of these resistant organisms.http://www.njmonline.org/article.asp?issn=1115-2613;year=2020;volume=29;issue=4;spage=701;epage=706;aulast=Ajubabeta-lactamasecarbapenemasenew-delhinigeriaoxacillinase
collection DOAJ
language English
format Article
sources DOAJ
author Ijeoma Maryrose Ajuba
Comfort Nne Akujobi
Iloduba Nnaemeka Aghanya
Simon Nkpeh Ushie
Akachukwu Egwu Okoro
Robinson Ogochukwu Ofiaeli
Chibuike Jesse Ezeama
Michel Chiedu Egbuniwe
Mabel Ogochukwu Okeke
spellingShingle Ijeoma Maryrose Ajuba
Comfort Nne Akujobi
Iloduba Nnaemeka Aghanya
Simon Nkpeh Ushie
Akachukwu Egwu Okoro
Robinson Ogochukwu Ofiaeli
Chibuike Jesse Ezeama
Michel Chiedu Egbuniwe
Mabel Ogochukwu Okeke
Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria
Nigerian Journal of Medicine
beta-lactamase
carbapenemase
new-delhi
nigeria
oxacillinase
author_facet Ijeoma Maryrose Ajuba
Comfort Nne Akujobi
Iloduba Nnaemeka Aghanya
Simon Nkpeh Ushie
Akachukwu Egwu Okoro
Robinson Ogochukwu Ofiaeli
Chibuike Jesse Ezeama
Michel Chiedu Egbuniwe
Mabel Ogochukwu Okeke
author_sort Ijeoma Maryrose Ajuba
title Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria
title_short Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria
title_full Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria
title_fullStr Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria
title_full_unstemmed Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria
title_sort emergence of new-delhi metallo-betalactamase-1 and oxacillinase-48 positive escherichia coli in south-eastern nigeria
publisher Wolters Kluwer Medknow Publications
series Nigerian Journal of Medicine
issn 1115-2613
publishDate 2020-01-01
description Objective: The spread of carbapenemase-producing Enterobacteriaceae is a global challenge that leads to an increase in health-care cost, treatment failures, high morbidity, and mortality. This study was aimed at determining the prevalence of New-Delhi Metallo Beta-Lactamase (NDM), and oxacillinase-48 (OXA-48) genes in clinical isolates of Escherichia coli obtained from a tertiary hospital in Nnewi, South-eastern, Nigeria. Materials and Methods: E. coli isolated from several clinical specimens including blood, urine, and wound swabs from patients receiving care at the hospital, were screened for resistance to meropenem and ertapenem antibiotics (Oxoid, UK) by the Kirby–Bauer disk-diffusion method. All isolates, which showed reduced sensitivity to the tested antibiotics, were then subjected to phenotypic confirmation of carbapenemase production using the Modified Hodge test. The NDM and OXA-48 genes were then detected using the polymerase chain reaction techniques. Results: Of the 187 E. coli isolates, 41 (21.9%) screened positive as suspected carbapenemase producers, while the prevalence of carbapenemase-producing E. coli in this study was 21/187 (11.23%). The prevalence of NDM and OXA-48 genes in the entire sample population was 3/187 (1.6%) and 12/187 (6.4%), respectively. Conclusion: The results obtained showed that NDM and OXA-48 carbapenemase-mediated resistance occurred in the study location. Hence, a reinforcement of infection prevention and control practices in the hospital will be required to curb the propagation of these resistant organisms.
topic beta-lactamase
carbapenemase
new-delhi
nigeria
oxacillinase
url http://www.njmonline.org/article.asp?issn=1115-2613;year=2020;volume=29;issue=4;spage=701;epage=706;aulast=Ajuba
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