Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria
Objective: The spread of carbapenemase-producing Enterobacteriaceae is a global challenge that leads to an increase in health-care cost, treatment failures, high morbidity, and mortality. This study was aimed at determining the prevalence of New-Delhi Metallo Beta-Lactamase (NDM), and oxacillinase-4...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2020-01-01
|
Series: | Nigerian Journal of Medicine |
Subjects: | |
Online Access: | http://www.njmonline.org/article.asp?issn=1115-2613;year=2020;volume=29;issue=4;spage=701;epage=706;aulast=Ajuba |
id |
doaj-d0d2cc21b92946a1934c98ff32d441c6 |
---|---|
record_format |
Article |
spelling |
doaj-d0d2cc21b92946a1934c98ff32d441c62021-08-09T10:22:56ZengWolters Kluwer Medknow PublicationsNigerian Journal of Medicine1115-26132020-01-0129470170610.4103/NJM.NJM_165_20Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern NigeriaIjeoma Maryrose AjubaComfort Nne AkujobiIloduba Nnaemeka AghanyaSimon Nkpeh UshieAkachukwu Egwu OkoroRobinson Ogochukwu OfiaeliChibuike Jesse EzeamaMichel Chiedu EgbuniweMabel Ogochukwu OkekeObjective: The spread of carbapenemase-producing Enterobacteriaceae is a global challenge that leads to an increase in health-care cost, treatment failures, high morbidity, and mortality. This study was aimed at determining the prevalence of New-Delhi Metallo Beta-Lactamase (NDM), and oxacillinase-48 (OXA-48) genes in clinical isolates of Escherichia coli obtained from a tertiary hospital in Nnewi, South-eastern, Nigeria. Materials and Methods: E. coli isolated from several clinical specimens including blood, urine, and wound swabs from patients receiving care at the hospital, were screened for resistance to meropenem and ertapenem antibiotics (Oxoid, UK) by the Kirby–Bauer disk-diffusion method. All isolates, which showed reduced sensitivity to the tested antibiotics, were then subjected to phenotypic confirmation of carbapenemase production using the Modified Hodge test. The NDM and OXA-48 genes were then detected using the polymerase chain reaction techniques. Results: Of the 187 E. coli isolates, 41 (21.9%) screened positive as suspected carbapenemase producers, while the prevalence of carbapenemase-producing E. coli in this study was 21/187 (11.23%). The prevalence of NDM and OXA-48 genes in the entire sample population was 3/187 (1.6%) and 12/187 (6.4%), respectively. Conclusion: The results obtained showed that NDM and OXA-48 carbapenemase-mediated resistance occurred in the study location. Hence, a reinforcement of infection prevention and control practices in the hospital will be required to curb the propagation of these resistant organisms.http://www.njmonline.org/article.asp?issn=1115-2613;year=2020;volume=29;issue=4;spage=701;epage=706;aulast=Ajubabeta-lactamasecarbapenemasenew-delhinigeriaoxacillinase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ijeoma Maryrose Ajuba Comfort Nne Akujobi Iloduba Nnaemeka Aghanya Simon Nkpeh Ushie Akachukwu Egwu Okoro Robinson Ogochukwu Ofiaeli Chibuike Jesse Ezeama Michel Chiedu Egbuniwe Mabel Ogochukwu Okeke |
spellingShingle |
Ijeoma Maryrose Ajuba Comfort Nne Akujobi Iloduba Nnaemeka Aghanya Simon Nkpeh Ushie Akachukwu Egwu Okoro Robinson Ogochukwu Ofiaeli Chibuike Jesse Ezeama Michel Chiedu Egbuniwe Mabel Ogochukwu Okeke Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria Nigerian Journal of Medicine beta-lactamase carbapenemase new-delhi nigeria oxacillinase |
author_facet |
Ijeoma Maryrose Ajuba Comfort Nne Akujobi Iloduba Nnaemeka Aghanya Simon Nkpeh Ushie Akachukwu Egwu Okoro Robinson Ogochukwu Ofiaeli Chibuike Jesse Ezeama Michel Chiedu Egbuniwe Mabel Ogochukwu Okeke |
author_sort |
Ijeoma Maryrose Ajuba |
title |
Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria |
title_short |
Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria |
title_full |
Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria |
title_fullStr |
Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria |
title_full_unstemmed |
Emergence of New-Delhi metallo-betalactamase-1 and oxacillinase-48 positive Escherichia coli in South-Eastern Nigeria |
title_sort |
emergence of new-delhi metallo-betalactamase-1 and oxacillinase-48 positive escherichia coli in south-eastern nigeria |
publisher |
Wolters Kluwer Medknow Publications |
series |
Nigerian Journal of Medicine |
issn |
1115-2613 |
publishDate |
2020-01-01 |
description |
Objective: The spread of carbapenemase-producing Enterobacteriaceae is a global challenge that leads to an increase in health-care cost, treatment failures, high morbidity, and mortality. This study was aimed at determining the prevalence of New-Delhi Metallo Beta-Lactamase (NDM), and oxacillinase-48 (OXA-48) genes in clinical isolates of Escherichia coli obtained from a tertiary hospital in Nnewi, South-eastern, Nigeria. Materials and Methods: E. coli isolated from several clinical specimens including blood, urine, and wound swabs from patients receiving care at the hospital, were screened for resistance to meropenem and ertapenem antibiotics (Oxoid, UK) by the Kirby–Bauer disk-diffusion method. All isolates, which showed reduced sensitivity to the tested antibiotics, were then subjected to phenotypic confirmation of carbapenemase production using the Modified Hodge test. The NDM and OXA-48 genes were then detected using the polymerase chain reaction techniques. Results: Of the 187 E. coli isolates, 41 (21.9%) screened positive as suspected carbapenemase producers, while the prevalence of carbapenemase-producing E. coli in this study was 21/187 (11.23%). The prevalence of NDM and OXA-48 genes in the entire sample population was 3/187 (1.6%) and 12/187 (6.4%), respectively. Conclusion: The results obtained showed that NDM and OXA-48 carbapenemase-mediated resistance occurred in the study location. Hence, a reinforcement of infection prevention and control practices in the hospital will be required to curb the propagation of these resistant organisms. |
topic |
beta-lactamase carbapenemase new-delhi nigeria oxacillinase |
url |
http://www.njmonline.org/article.asp?issn=1115-2613;year=2020;volume=29;issue=4;spage=701;epage=706;aulast=Ajuba |
work_keys_str_mv |
AT ijeomamaryroseajuba emergenceofnewdelhimetallobetalactamase1andoxacillinase48positiveescherichiacoliinsoutheasternnigeria AT comfortnneakujobi emergenceofnewdelhimetallobetalactamase1andoxacillinase48positiveescherichiacoliinsoutheasternnigeria AT ilodubannaemekaaghanya emergenceofnewdelhimetallobetalactamase1andoxacillinase48positiveescherichiacoliinsoutheasternnigeria AT simonnkpehushie emergenceofnewdelhimetallobetalactamase1andoxacillinase48positiveescherichiacoliinsoutheasternnigeria AT akachukwuegwuokoro emergenceofnewdelhimetallobetalactamase1andoxacillinase48positiveescherichiacoliinsoutheasternnigeria AT robinsonogochukwuofiaeli emergenceofnewdelhimetallobetalactamase1andoxacillinase48positiveescherichiacoliinsoutheasternnigeria AT chibuikejesseezeama emergenceofnewdelhimetallobetalactamase1andoxacillinase48positiveescherichiacoliinsoutheasternnigeria AT michelchieduegbuniwe emergenceofnewdelhimetallobetalactamase1andoxacillinase48positiveescherichiacoliinsoutheasternnigeria AT mabelogochukwuokeke emergenceofnewdelhimetallobetalactamase1andoxacillinase48positiveescherichiacoliinsoutheasternnigeria |
_version_ |
1721214303233638400 |