Square Wave Voltammetric Determination of 2-Thiouracil in Pharmaceuticals and Real Samples Using Glassy Carbon Electrode

• Main Authors: Naveen M. Gokavi, Vijay P. Pattar, Atmanand M. Bagoji, Sharanappa T. Nandibewoor
• Format: Article
• Language: English
• Published: Hindawi Limited 2013-01-01
• Series: International Journal of Electrochemistry
• Online Access: http://dx.doi.org/10.1155/2013/627854

A simple and rapid method was developed using cyclic and square wave voltammetric techniques for the determination of trace-level sulfur containing compound, 2-thiouracil, at a glassy carbon electrode. 2-thiouracil produced two anodic peaks at 0.334 V and 1.421 V and a cathodic peak at −0.534 V. The square wave voltammetry of 2-thiouracil gave a good linear response in the range of 1–20 μM with a detection limit of 0.16 μM and quantification limit of 0.53 μM (0.0679 μg/g), which is in good agreement as per IUPAC definition of trace component analysis (100 μg/g). The obtained recoveries range from 98.10% to 102.1%. The proposed method was used successfully for its quantitative determination in pharmaceutical formulations and urine as real samples.