Genomic Analysis of the Recent Viral Isolate vB_BthP-Goe4 Reveals Increased Diversity of φ29-Like Phages

Genomic Analysis of the Recent Viral Isolate vB_BthP-Goe4 Reveals Increased Diversity of φ29-Like Phages

We present the recently isolated virus vB_BthP-Goe4 infecting <i>Bacillus thuringiensis</i> HD1. Morphological investigation via transmission electron microscopy revealed key characteristics of the genus <i>Phi29virus</i>, but with an elongated head resulting in larger virion...

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Bibliographic Details
Main Authors: Tobias Schilling, Michael Hoppert, Robert Hertel
Format: Article
Language:English
Published: MDPI AG 2018-11-01
Series:Viruses
Subjects:
<i>Bacillus</i>
thuringiensis
vB_BthP-Goe4
Goe4
Picovirinae
Phi29virus
<i>Salasvirus</i>
Luci
bacteriophage
phage
pRNA
Online Access:https://www.mdpi.com/1999-4915/10/11/624
Description
Summary:We present the recently isolated virus vB_BthP-Goe4 infecting <i>Bacillus thuringiensis</i> HD1. Morphological investigation via transmission electron microscopy revealed key characteristics of the genus <i>Phi29virus</i>, but with an elongated head resulting in larger virion particles of approximately 50 nm width and 120 nm height. Genome sequencing and analysis resulted in a linear phage chromosome of approximately 26 kb, harbouring 40 protein-encoding genes and a packaging RNA. Sequence comparison confirmed the relation to the <i>Phi29virus</i> genus and genomes of other related strains. A global average nucleotide identity analysis of all identified &#966;29-like viruses revealed the formation of several new groups previously not observed. The largest group includes Goe4 and may significantly expand the genus <i>Phi29virus</i> (<i>Salasvirus</i>) or the <i>Picovirinae</i> subfamily.
ISSN:1999-4915

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