Green fluorescent protein purification through Immobilized Metal Affinity Chromatografy (IMAC) and its relevance for Biomedical Science students during Biochemistry practical classes at La Trobe University – Australia

This work was performed as an integrated practical of a Biomedical Science undergraduate course of Biochemistry subject, in order to demonstrate used techniques to purify of Green Fluorescent Protein (GFP). To perform the experiments the main methodology applied was the by immobilized metal affinity...

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Bibliographic Details
Main Authors: Alex Jose José de Melo Silva, Lumar Lucena Alves, Julian Pakay
Format: Article
Language:Portuguese
Published: Sociedade Brasileira de Bioquímica e Biologia Molecular, SBBq 2016-12-01
Series:Journal of Biochemistry Education
Subjects:
GFP
Online Access:http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/638