Development of Glutathione Production Technology Based on Constructed Active Yeast Overproducers

• Main Authors: Yurkiv M.T., Kurylenko O.O., Vasylyshyn R.V., Dmytruk K.V., Martynyuk N.B., Skorohod V.V., Sybirny A.A.
• Format: Article
• Language: Ukrainian
• Published: Publishing House "Akademperiodyka" 2015-09-01
• Series: Nauka ta Innovacii
• Subjects: glutathione; yeast; Hansenula polymorpha; metabolic engineering.; metabolic engineering
• Online Access: ftp://nas.gov.ua/akademperiodyka/Downloads/Archive%20SI%20Journal/SI_ukr/2015/N5/Yurkiv.pdf
• ISSN: 1815-2066; 2413-497X

Recombinant Hansenula polymorpha strain overexpressing both GSH2 gene, encoding γ-glutamylcysteine synthetase, and MET4 gene, coding for transcription activator of genes involved in cysteine biosynthesis (precursor of glutathione) was obtained applying metabolic engineering approaches. Obtained recombinant strain was characterized by significantly increased glutathione production as compared to the wild type strain in laboratory conditions. Conditions for efficient glutathione production by recombinant H. polymorpha strain were optimized. A semi-industrial model for glutathione production using constructed H. polymorpha overproducer was developed.