Development and Characterization of a Novel Anti-idiotypic Monoclonal Antibody to Growth Hormone, Which Can Mimic Physiological Functions of Growth Hormone in Primary Porcine Hepatocytes

• Main Authors: Hai-Nan Lan, Hai-Long Jiang, Wei Li, Tian-Cheng Wu, Pan Hong, Yu Meng Li, Hui Zhang, Huan-Zhong Cui, Xin Zheng
• Format: Article
• Language: English
• Published: Asian-Australasian Association of Animal Production Societies 2015-04-01
• Series: Asian-Australasian Journal of Animal Sciences
• Subjects: Porcine Growth Hormone; Monoclonal Anti-idiotypic Antibodies (Mab2s); Porcine Hepatocytes; Janus Kinase (2)-Signal Transducers and Activators of Transcription [JAK2-STAT5]
• Online Access: http://www.ajas.info/upload/pdf/ajas-28-4-573.pdf
• ISSN: 1011-2367; 1976-5517

B-32 is one of a panel of monoclonal anti-idiotypic antibodies to growth hormone (GH) that we developed. To characterize and identify its potential role as a novel growth hormone receptor (GHR) agonist, we determined that B-32 behaved as a typical Ab2β based on a series of enzyme-linked immunosorbent assay assays. The results of fluorescence-activated cell sorting, indirect immunofluorescence and competitive receptor binding assays demonstrated that B-32 specifically binds to the GHR expressed on target cells. Next, we examined the resulting signal transduction pathways triggered by this antibody in primary porcine hepatocytes. We found that B-32 can activate the GHR and Janus kinase (2)/signal transducers and activators of transcription (JAK2/STAT5) signalling pathways. The phosphorylation kinetics of JAK2/STAT5 induced by either GH or B-32 were analysed in dose-response and time course experiments. In addition, B32 could also stimulate porcine hepatocytes to secrete insulin-like growth factors-1. Our work indicates that a monoclonal anti-idiotypic antibody to GH (B-32) can serve as a GHR agonist or GH mimic and has application potential in domestic animal (pig) production.