Synthesis of Oligonucleotide Conjugates and Phosphorylated Nucleotide Analogues: An Improvement to a Solid Phase Synthetic Approach

An improvement to our solid phase strategy to generate pharmacologically interesting molecule libraries is proposed here. The synthesis of new o-chlorophenol-functionalised solid supports with very high loading (0.18–0.22 meq/g for control pore glass (CPG) and 0.25–0.50 meq/g for TG) is reported. To...

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Main Authors:	Valeria Romanucci, Armando Zarrelli, Lorenzo De Napoli, Cinzia Di Marino, Giovanni Di Fabio
Format:	Article
Language:	English
Published:	Hindawi Limited 2013-01-01
Series:	Journal of Chemistry
Online Access:	http://dx.doi.org/10.1155/2013/469470

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spelling	doaj-d027bdac7ca345e09eea4a230b8e25422020-11-24T22:26:51ZengHindawi LimitedJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/469470469470Synthesis of Oligonucleotide Conjugates and Phosphorylated Nucleotide Analogues: An Improvement to a Solid Phase Synthetic ApproachValeria Romanucci0Armando Zarrelli1Lorenzo De Napoli2Cinzia Di Marino3Giovanni Di Fabio4Department of Chemical Sciences, University of Napoli “Federico II”, Via Cintia 4, 80126 Napoli, ItalyDepartment of Chemical Sciences, University of Napoli “Federico II”, Via Cintia 4, 80126 Napoli, ItalyDepartment of Chemical Sciences, University of Napoli “Federico II”, Via Cintia 4, 80126 Napoli, ItalyDepartment of Chemical Sciences, University of Napoli “Federico II”, Via Cintia 4, 80126 Napoli, ItalyDepartment of Chemical Sciences, University of Napoli “Federico II”, Via Cintia 4, 80126 Napoli, ItalyAn improvement to our solid phase strategy to generate pharmacologically interesting molecule libraries is proposed here. The synthesis of new o-chlorophenol-functionalised solid supports with very high loading (0.18–0.22 meq/g for control pore glass (CPG) and 0.25–0.50 meq/g for TG) is reported. To test the efficiency of these supports, we prepared nucleotide and oligonucleotide models, and their coupling yields and the purity of the crude detached materials were comparable to previously available results. These supports allow the facile and high-yield preparation of highly pure phosphodiester and phosphoramidate monoester nucleosides, conjugated oligonucleotides, and other yet unexplored classes of phosphodiester and phosphoramidate molecules.http://dx.doi.org/10.1155/2013/469470
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Valeria Romanucci Armando Zarrelli Lorenzo De Napoli Cinzia Di Marino Giovanni Di Fabio
spellingShingle	Valeria Romanucci Armando Zarrelli Lorenzo De Napoli Cinzia Di Marino Giovanni Di Fabio Synthesis of Oligonucleotide Conjugates and Phosphorylated Nucleotide Analogues: An Improvement to a Solid Phase Synthetic Approach Journal of Chemistry
author_facet	Valeria Romanucci Armando Zarrelli Lorenzo De Napoli Cinzia Di Marino Giovanni Di Fabio
author_sort	Valeria Romanucci
title	Synthesis of Oligonucleotide Conjugates and Phosphorylated Nucleotide Analogues: An Improvement to a Solid Phase Synthetic Approach
title_short	Synthesis of Oligonucleotide Conjugates and Phosphorylated Nucleotide Analogues: An Improvement to a Solid Phase Synthetic Approach
title_full	Synthesis of Oligonucleotide Conjugates and Phosphorylated Nucleotide Analogues: An Improvement to a Solid Phase Synthetic Approach
title_fullStr	Synthesis of Oligonucleotide Conjugates and Phosphorylated Nucleotide Analogues: An Improvement to a Solid Phase Synthetic Approach
title_full_unstemmed	Synthesis of Oligonucleotide Conjugates and Phosphorylated Nucleotide Analogues: An Improvement to a Solid Phase Synthetic Approach
title_sort	synthesis of oligonucleotide conjugates and phosphorylated nucleotide analogues: an improvement to a solid phase synthetic approach
publisher	Hindawi Limited
series	Journal of Chemistry
issn	2090-9063 2090-9071
publishDate	2013-01-01
description	An improvement to our solid phase strategy to generate pharmacologically interesting molecule libraries is proposed here. The synthesis of new o-chlorophenol-functionalised solid supports with very high loading (0.18–0.22 meq/g for control pore glass (CPG) and 0.25–0.50 meq/g for TG) is reported. To test the efficiency of these supports, we prepared nucleotide and oligonucleotide models, and their coupling yields and the purity of the crude detached materials were comparable to previously available results. These supports allow the facile and high-yield preparation of highly pure phosphodiester and phosphoramidate monoester nucleosides, conjugated oligonucleotides, and other yet unexplored classes of phosphodiester and phosphoramidate molecules.
url	http://dx.doi.org/10.1155/2013/469470
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Synthesis of Oligonucleotide Conjugates and Phosphorylated Nucleotide Analogues: An Improvement to a Solid Phase Synthetic Approach

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