Fast Fabrication of Solid-State Nanopores for DNA Molecule Analysis

Fast Fabrication of Solid-State Nanopores for DNA Molecule Analysis

Solid-state nanopores have been developed as a prominent tool for single molecule analysis in versatile applications. Although controlled dielectric breakdown (CDB) is the most accessible method for a single nanopore fabrication, it is still necessary to improve the fabrication efficiency and avoid...

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Main Authors: Yin Zhang, Dexian Ma, Zengdao Gu, Lijian Zhan, Jingjie Sha
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Nanomaterials
Subjects:
solid-state nanopore
dielectric breakdown
biosensor
DNA detection
Online Access:https://www.mdpi.com/2079-4991/11/9/2450
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spelling doaj-d01b61dacf54493f8f33c3938ae55aba2021-09-26T00:49:39ZengMDPI AGNanomaterials2079-49912021-09-01112450245010.3390/nano11092450Fast Fabrication of Solid-State Nanopores for DNA Molecule AnalysisYin Zhang0Dexian Ma1Zengdao Gu2Lijian Zhan3Jingjie Sha4Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, ChinaJiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, ChinaJiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, ChinaJiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, ChinaJiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, ChinaSolid-state nanopores have been developed as a prominent tool for single molecule analysis in versatile applications. Although controlled dielectric breakdown (CDB) is the most accessible method for a single nanopore fabrication, it is still necessary to improve the fabrication efficiency and avoid the generation of multiple nanopores. In this work, we treated the SiNx membranes in the air–plasma before the CDB process, which shortened the time-to-pore-formation by orders of magnitude. λ-DNA translocation experiments validated the functionality of the pore and substantiated the presence of only a single pore on the membrane. Our fabricated pore could also be successfully used to detect short single-stranded DNA (ssDNA) fragments. Using to ionic current signals, ssDNA fragments with different lengths could be clearly distinguished. These results will provide a valuable reference for the nanopore fabrication and DNA analysis.https://www.mdpi.com/2079-4991/11/9/2450solid-state nanoporedielectric breakdownbiosensorDNA detection
collection DOAJ
language English
format Article
sources DOAJ
author Yin Zhang
Dexian Ma
Zengdao Gu
Lijian Zhan
Jingjie Sha
spellingShingle Yin Zhang
Dexian Ma
Zengdao Gu
Lijian Zhan
Jingjie Sha
Fast Fabrication of Solid-State Nanopores for DNA Molecule Analysis
Nanomaterials
solid-state nanopore
dielectric breakdown
biosensor
DNA detection
author_facet Yin Zhang
Dexian Ma
Zengdao Gu
Lijian Zhan
Jingjie Sha
author_sort Yin Zhang
title Fast Fabrication of Solid-State Nanopores for DNA Molecule Analysis
title_short Fast Fabrication of Solid-State Nanopores for DNA Molecule Analysis
title_full Fast Fabrication of Solid-State Nanopores for DNA Molecule Analysis
title_fullStr Fast Fabrication of Solid-State Nanopores for DNA Molecule Analysis
title_full_unstemmed Fast Fabrication of Solid-State Nanopores for DNA Molecule Analysis
title_sort fast fabrication of solid-state nanopores for dna molecule analysis
publisher MDPI AG
series Nanomaterials
issn 2079-4991
publishDate 2021-09-01
description Solid-state nanopores have been developed as a prominent tool for single molecule analysis in versatile applications. Although controlled dielectric breakdown (CDB) is the most accessible method for a single nanopore fabrication, it is still necessary to improve the fabrication efficiency and avoid the generation of multiple nanopores. In this work, we treated the SiNx membranes in the air–plasma before the CDB process, which shortened the time-to-pore-formation by orders of magnitude. λ-DNA translocation experiments validated the functionality of the pore and substantiated the presence of only a single pore on the membrane. Our fabricated pore could also be successfully used to detect short single-stranded DNA (ssDNA) fragments. Using to ionic current signals, ssDNA fragments with different lengths could be clearly distinguished. These results will provide a valuable reference for the nanopore fabrication and DNA analysis.
topic solid-state nanopore
dielectric breakdown
biosensor
DNA detection
url https://www.mdpi.com/2079-4991/11/9/2450
work_keys_str_mv AT yinzhang fastfabricationofsolidstatenanoporesfordnamoleculeanalysis
AT dexianma fastfabricationofsolidstatenanoporesfordnamoleculeanalysis
AT zengdaogu fastfabricationofsolidstatenanoporesfordnamoleculeanalysis
AT lijianzhan fastfabricationofsolidstatenanoporesfordnamoleculeanalysis
AT jingjiesha fastfabricationofsolidstatenanoporesfordnamoleculeanalysis
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