| Summary: | Abstract Two bacteria, namely Bacillus subtilis and Pseudomonas aeruginosa were exposed to different concentrations of diesel to increase their adaptation to the fossil fuel, and were used for the degradation of diesel-biodiesel blends. The biodegradation rate was evaluated using the redox indicator 2,6-dichlorophenol indophenol (DCPIP) test and gravimetric analysis. The preliminary exposure of cells to diesel proved to improve their biodegradation capacities, and exposure to a higher concentration (70%) of diesel resulted in maximum biodegradation of up to 58.38 g and 1.78 g of degraded oil per CFU/mL (1010) for P. aeruginosa and B. subtilis respectively. It was found that the adapted cells preferably used diesel in the blend. P. aeruginosa and B. subtilis exhibited different adaptation capabilities and biodegradation behaviour. Biodiesel stimulated the biodegradation of the diesel-biodiesel blends by non-adapted cells only; the adapted cells exhibited a different behaviour.
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