Evaluation of synergistic therapeutic effect of shark cartilage extract with artemisinin and glucantime on visceral leishmaniasis in BALB/c mice

• Main Authors: Soheila Molaie, Fatemeh Ghafarifar, Abdolhossein Dalimi, Zoheir Hassan, Zohreh Sharifi
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2019-02-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: Artemisinin; BALB/c mice; Glucantim; Shark cartilage extract; Synergism; Visceral leishmaniasis
• Online Access: http://ijbms.mums.ac.ir/article_11817_f0f5fc65e44cae0e0892a099e520dd49.pdf

Objective(s): Because leishmaniasis is related to the impaired functioning of T-cells, the use of an immunomodulator can increase the efficacy of antileishmanial therapy in visceral leishmaniasis. In this study, we used shark cartilage extract with artemisinin and glucantime against visceral leishmaniasis in BALB/c mice, and evaluated the synergistic therapeutic effect. Materials and Methods: The culturing method and quantitative real-time PCR by using the kDNA gene was used to detect parasite loads in the spleen and liver. INF-γ and IL-4 cytokine levels and survival rates were assayed.Results: The drug therapy with target drugs reduced parasite burden in the spleen and liver significantly. Although parasite burden was lower in the artemisinin treated group than in the glucantime treated group (P