Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells

• Main Authors: Keyong Xu, Feng Liu, Benguo Liu, Han Gao, Zhengxiang Ning
• Format: Article
• Language: English
• Published: MDPI AG 2010-04-01
• Series: International Journal of Molecular Sciences
• Subjects: apoptosis; cell cycle; Hep G2; 4′,7-diacetoxyapigenin
• Online Access: http://www.mdpi.com/1422-0067/11/5/1991/

In this study, 4′,7-diacetoxyapigenin [4-(7-acetoxy-5-hydroxy-4-oxo-4H-chromen-2-yl) phenyl acetate] was synthesized for the first time. Its chemical structure was identified by UV, ESI-MS, 1H and 13C-NMR. It could inhibit the proliferation of Hep G2 cells in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treatment by 4′,7-diacetoxyapigenin, phosphatidylserine of Hep G2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-FITC and PI staining. It was concluded that 4′,7-diacetoxyapigenin not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle.