On the possibility of PhotoEmission Electron Microscopy for E. coli advanced studies

The novel approach was proposed for detailed high-resolution studies of morphology and physico-chemical properties concomitantly at one measurement spot of E. coli bacterial cells culture immobilized onto silicon wafer surface in UHV conditions applying PhotoEmission Electron Microscopy under Hg lam...

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Main Authors:	S.Yu. Turishchev, D. Marchenko, V. Sivakov, E.A. Belikov, O.A. Chuvenkova, E.V. Parinova, D.A. Koyuda, R.G. Chumakov, A.M. Lebedev, T.V. Kulikova, A.A. Berezhnoy, I.V. Valiakhmedova, N.V. Praslova, E.V. Preobrazhenskaya, S.S. Antipov
Format:	Article
Language:	English
Published:	Elsevier 2020-03-01
Series:	Results in Physics
Subjects:	Spectromicroscopy Photoemission electron microscopy Scanning electron microscopy X-ray photoelectron spectroscopy Surface composition Bioimaging
Online Access:	http://www.sciencedirect.com/science/article/pii/S2211379719331869

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record_format	Article
spelling	doaj-cf929ef95d5a410b84594c6723978ef02020-11-25T02:23:06ZengElsevierResults in Physics2211-37972020-03-0116102821On the possibility of PhotoEmission Electron Microscopy for E. coli advanced studiesS.Yu. Turishchev0D. Marchenko1V. Sivakov2E.A. Belikov3O.A. Chuvenkova4E.V. Parinova5D.A. Koyuda6R.G. Chumakov7A.M. Lebedev8T.V. Kulikova9A.A. Berezhnoy10I.V. Valiakhmedova11N.V. Praslova12E.V. Preobrazhenskaya13S.S. Antipov14Voronezh State University, 394018, Universitetskaya pl.1, Voronezh, Russia; Corresponding author.Helmholtz-Zentrum Berlin, 12489, Albert-Einstein-Str. 15, Berlin, GermanyLeibniz Institute of Photonic Technology, 07745, Albert-Einstein-Str. 9, Jena, GermanyVoronezh State University, 394018, Universitetskaya pl.1, Voronezh, RussiaVoronezh State University, 394018, Universitetskaya pl.1, Voronezh, RussiaVoronezh State University, 394018, Universitetskaya pl.1, Voronezh, RussiaVoronezh State University, 394018, Universitetskaya pl.1, Voronezh, RussiaNational Research Center “Kurchatov Institute”, 123182, Akademika Kurchatova pl. 1, Moscow, RussiaNational Research Center “Kurchatov Institute”, 123182, Akademika Kurchatova pl. 1, Moscow, RussiaVoronezh State University, 394018, Universitetskaya pl.1, Voronezh, RussiaVoronezh State University, 394018, Universitetskaya pl.1, Voronezh, RussiaVoronezh State University, 394018, Universitetskaya pl.1, Voronezh, RussiaVoronezh State University, 394018, Universitetskaya pl.1, Voronezh, RussiaInstitute of Cell Biophysics RAS, 142290, Institutskaya St. 3, Pushchino, Moskovskaya obl., RussiaVoronezh State University, 394018, Universitetskaya pl.1, Voronezh, Russia; Institute of Cell Biophysics RAS, 142290, Institutskaya St. 3, Pushchino, Moskovskaya obl., Russia; Immanuel Kant Baltic Federal University, 236016, A. Nevskogo St. 14, Kaliningrad, RussiaThe novel approach was proposed for detailed high-resolution studies of morphology and physico-chemical properties concomitantly at one measurement spot of E. coli bacterial cells culture immobilized onto silicon wafer surface in UHV conditions applying PhotoEmission Electron Microscopy under Hg lamp irradiation. For the E. coli characterization scanning electron microscopy (electron beam) and X-ray photoelectron spectroscopy (X-ray tube radiation) were applied prior to PhotoEmission Electron Microscopy measurements. In spite of irradiation doses collected for the cell arrays we were successful in detection of high-resolution images even of single E. coli bacterium by PhotoEmission Electron Microscopy technique followed by detailed high-resolution morphology studies by scanning electron microscopy. These results revealed widespread stability of the E. coli membranes shape after the significant number of applied characterization techniques.http://www.sciencedirect.com/science/article/pii/S2211379719331869SpectromicroscopyPhotoemission electron microscopyScanning electron microscopyX-ray photoelectron spectroscopySurface compositionBioimaging
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	S.Yu. Turishchev D. Marchenko V. Sivakov E.A. Belikov O.A. Chuvenkova E.V. Parinova D.A. Koyuda R.G. Chumakov A.M. Lebedev T.V. Kulikova A.A. Berezhnoy I.V. Valiakhmedova N.V. Praslova E.V. Preobrazhenskaya S.S. Antipov
spellingShingle	S.Yu. Turishchev D. Marchenko V. Sivakov E.A. Belikov O.A. Chuvenkova E.V. Parinova D.A. Koyuda R.G. Chumakov A.M. Lebedev T.V. Kulikova A.A. Berezhnoy I.V. Valiakhmedova N.V. Praslova E.V. Preobrazhenskaya S.S. Antipov On the possibility of PhotoEmission Electron Microscopy for E. coli advanced studies Results in Physics Spectromicroscopy Photoemission electron microscopy Scanning electron microscopy X-ray photoelectron spectroscopy Surface composition Bioimaging
author_facet	S.Yu. Turishchev D. Marchenko V. Sivakov E.A. Belikov O.A. Chuvenkova E.V. Parinova D.A. Koyuda R.G. Chumakov A.M. Lebedev T.V. Kulikova A.A. Berezhnoy I.V. Valiakhmedova N.V. Praslova E.V. Preobrazhenskaya S.S. Antipov
author_sort	S.Yu. Turishchev
title	On the possibility of PhotoEmission Electron Microscopy for E. coli advanced studies
title_short	On the possibility of PhotoEmission Electron Microscopy for E. coli advanced studies
title_full	On the possibility of PhotoEmission Electron Microscopy for E. coli advanced studies
title_fullStr	On the possibility of PhotoEmission Electron Microscopy for E. coli advanced studies
title_full_unstemmed	On the possibility of PhotoEmission Electron Microscopy for E. coli advanced studies
title_sort	on the possibility of photoemission electron microscopy for e. coli advanced studies
publisher	Elsevier
series	Results in Physics
issn	2211-3797
publishDate	2020-03-01
description	The novel approach was proposed for detailed high-resolution studies of morphology and physico-chemical properties concomitantly at one measurement spot of E. coli bacterial cells culture immobilized onto silicon wafer surface in UHV conditions applying PhotoEmission Electron Microscopy under Hg lamp irradiation. For the E. coli characterization scanning electron microscopy (electron beam) and X-ray photoelectron spectroscopy (X-ray tube radiation) were applied prior to PhotoEmission Electron Microscopy measurements. In spite of irradiation doses collected for the cell arrays we were successful in detection of high-resolution images even of single E. coli bacterium by PhotoEmission Electron Microscopy technique followed by detailed high-resolution morphology studies by scanning electron microscopy. These results revealed widespread stability of the E. coli membranes shape after the significant number of applied characterization techniques.
topic	Spectromicroscopy Photoemission electron microscopy Scanning electron microscopy X-ray photoelectron spectroscopy Surface composition Bioimaging
url	http://www.sciencedirect.com/science/article/pii/S2211379719331869
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On the possibility of PhotoEmission Electron Microscopy for E. coli advanced studies

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